Radiology is strongly depending on medical imaging technology and consequently directing technological progress. A novel technology can only be established, however, if improved diagnostic accuracy influence on therapeutic management and/or overall reduced cost can be evidenced.

We carried out an analysis on the genomic organisation of the tRNA(Glu) family in S. cerevisiae; eight clones were characterized by restriction mapping, hybridization and sequencing. These data taken together with our earlier findings show that the individual tRNA(Glu3) copies are identical only in their structural part but embedded in entirely different genomic environments. All of the tRNA genes identified here are flanked by elements such as Ty, delta, sigma, and tau. In some cases, sequences from different elements form complex patterns indicating a sophisticated history of these chromosomal regions. A novel observation is that Ty and delta in the regions analyzed are exclusively associated with the tRNA genes. The observed patterns imply that the tRNA genes mark regions of multiple transposition and subsequent excision events, but that these have occurred after the individual tRNA gene copies had been fixed in their present locations. Transcription experiments by the use of micro-injection into Xenopus oocytes suggest that the elements flanking the tRNA genes exert a modulating effect on their expression.

Siemens recently launched a new product, the MAGNETOM Avanto, onto the market. The initial customer reaction was tremendous. The product definition followed a structured process and state‐of‐the‐art tools like quality function deployment (QFD) and conjoint analysis were applied. This paper analyses the impact of all these efforts in product definition on market success. It retrospectively summarizes the benefits from various tools and methods. The medical equipment market, its mechanisms and special features are introduced in a case study of magnetic resonance imaging equipment developed at Siemens Medical Solutions. The decision‐making or purchasing process in this market is very complex. Therefore dedicated market research approaches using a variety of different methods were required. Focus groups, conjoint analysis, broadband surveys and face‐to‐face interviews were all useful. In addition, an astute segmentation of the individual groups that influence a purchasing decision had to be made. From all these market research activities a company normally ends up with an immense amount of contradicting requirements. The article shows how these requirements were converted into a clear system specification and new technological solutions. The Siemens MAGNETOM Avanto has been very successful in the market. Customer reaction and feedback indicates that the product has many unique selling points, best performance in many aspects, and no major weaknesses. Siemens believes that this is due to the very systematic and comprehensive approach of the product definition.

This is the accepted version of the paper.This version of the publication may differ from the final published version. Permanent repository link ABSTRACTAccurate staging of nodal cancer still relies on surgical exploration because many primary malignancies spread via lymphatic dissemination. The purpose of this study was to utilize nanoparticle-enhanced lymphotropic magnetic resonance imaging (LN-MRI) to explore semi-automated noninvasive nodal cancer staging. We present a joint image segmentation and registration approach, which makes use of the problem specific information to increase the robustness of the algorithm to noise and weak contrast often observed in medical imaging applications. The effectiveness of the approach is demonstrated with a given lymph node segmentation problem in post-contrast pelvic MRI sequences.

To monitor in vivo transcription and chromatin structure of yeast tRNA genes, we constructed a synthetic tRNA gene that can be used as a reporter. Constructs in which this synthetic tRNA gene is combined with different flanking regions can be integrated into the genome as single copies. The artificial tRNA gene is tagged by the insertion of an intron-like sequence that cannot be spliced out from the precursor and transcripts can thus be identified and quantitated. By several criteria, the artificial tRNA gene behaves like a resident tRNA gene. By measuring the accessibility towards DNaseI in chromatin, we found that the artificial tRNA gene exhibits the same characteristic pattern as resident tRNA genes. Three DNaseI-sensitive sites across the transcribed part of the gene and the immediate flanking regions reflect the formation of the stable transcription complex; positioned nucleosomes are observed in the upstream flanking region. We are confident that the system we have established will prove useful for studying regulatory aspects of tRNA gene expression as well as aspects of pre-tRNA processing and splicing.