Isolation and morphology of temperate Agrobacterium tumefaciens bacteriophage. J. Bacteriol. 92:746-750. 1966.-Lysogeny was detected in 14 strains of Agrobacterium tumefaciens among 130 bacterial strains tested with strain B-6 used as the host. Partial lysis was observed with 13 additional bacterial strains. Morphological studies of five strains showed that the phage had similar features. A typical phage (Lv-1) had a polyhedralshaped head, approximately 71 by 63 my, and a tail, approximately 211 m,t by 9.5 m,u. The phage nucleic acid was found to be deoxyribonucleic acid. The bacteriophage have been designated L (for lysogenic) followed by the bacterial strain designation in lower case letters. A temperate bacteriophage (w) was isolated from Agrobacterium tumefaciens (strain B-6) by Beardsley in 1955 (2). He used a phage-sensitive isolate of B-6, which had been obtained by ultraviolet irradiation, as the host. Other strains of A. tumefaciens tested, by Beardsley, as hosts for the w phage, however, failed to show zones of lysis (3). Recently J. McSharry and T. Stonier (personal communication) detected a temperate phage in A. tumefaciens B-2. This phage (PB-2) caused lysis of strains B-6, B6-G6, and a derivative of B-2.

No abstract

No abstract