789during the 9-day 'culture period. 2. The chorioallantois. 3. Thme tumor does not average percentage of successful grafts is in-metastasize from the chorioallantois in spite creased by implantation two or more pieces of apparent direct conitact between the emof tumor within a single egg, and by incubat-bryonic blood and the tumor cells. ing lthe for hours in Received June 28, 1950. P.S.E.B.M., 1950, ~7 4 ,rabbit serum blefore implantation onto the Use

Fertility and potential fertility were evaluated in 9 young men on cyclosporine A therapy following renal transplantation. Semen analysis was normal in most parameters in 8 patients as was testicular hormonal function. Of 4 men who had attempted to impregnate their wives 3 succeeded. Cyclosporine A does not seem to affect adversely fertility in men.

The physiological defects of salt and water retention appear to be the same in diseases of various etiologies associated with edema. However, the relative amounts of sodium and water retained and their distribution may be different and related to the metabolic faults of the particular disease. Although the concentrations of electrolytes in extracellular fluid are similar in edematous states, the concentrations in other compartments may indicate differences related to the relative accumulation and distribution of sodium and water.At present, methods are available to determine the concentrations of electrolytes in extracellular fluid. In intact man, concentrations of ions in other compartments of the body have to be indirectly determined. One such assay may be attempted by computing the body concentration of the electrolyte from the determinations of total exchangeable body content of the electrolyte and total body water. The value so derived does not have the physical chemical significance of concentration but may be indicative of changes occurring in the extracellular fluid as well as in other body compartments.Employing this procedure, the present study attempts to differentiate various clinical conditions with edema on the basis of total exchangeable sodium and body water content. In particular, patients with congestive heart failure have been compared to patients with other diseases associated with edema. The data indicate that patients with congestive heart failure apparently have a high body concentration of sodium (mEq. of exchangeable sodium per liter of body water).

Present methods of management of juvenile-onset diabetes mellitus do not prevent serious and debilitating complications affecting multiple organ systems. In an effort to reverse advanced forms of these complications, segmental transplantation of the pancreas has been performed on 10 patients, seven of whom simultaneously or subsequently received renal transplants. Long periods of normoglycemia (two to four and one-half years) were achieved in two patients who also maintained transplant kidney function. The course of these two patients is described to illustrate the possible value and limitations of the procedure. These patients had normal blood glucose levels, exhibited repeated normal intravenous glucose tolerance curves, and had repeated normal endogenous insulin levels. Their courses were characterized by (1) absence of problems related to pancreatic exocrine secretions into the bladder; (2) stable eye changes despite some episodes of hemorrhage from preexisting retinopathy; (3) vascular complications, including stroke and gangrene of extremities necessitating amputation despite successful femoropopliteal bypass grafting; (4) peripheral neuropathy; and (5) repeated infections. Both patients succumbed to vascular complications. Thus, pancreatic transplantation can maintain blood glucose and insulin at normal levels for extended periods of time. However, it does not reverse such complications as advanced retinopathy or atherosclerosis. Since the procedure may have value in preventing progression of these complications, it should be evaluated in patients with less advanced complications of diabetes.