To evaluate the therapeutic contribution of MSC intravenous infusion to surgical wound healing in dexamethasone-immunosuppressed rats, thirty-five rats were randomly divided into 2 groups: in the Control Group (CG), five rats received normal saline as 0.2ml subcutaneous (SC) injections every 24 hours, for 30 consecutive days and, in the Dexamethasone Group (DG), 30 rats were given 0.2mL subcutaneous dexamethasone (0.1mg kg-1) every 24 hours, for 30 consecutive days. After 30 days, all rats underwent surgery to create an experimental skin wound. The 30 animals of the DG group were divided into two equal groups, which received different treatments: the dexamethasone group (DG) received a single application of 0.5ml normal saline, via the intravenous route (IV), 48 hours after wound creation; and the Mesenchymal Stem Cells Dexamethasone group (MSCDG) received MSC transplantation at a concentration of 9x106 cells in a single IV application, 48 hours after wound creation. The surgical wounds of CG rats closed on average 14.75 days after creation and DG rats had wounds closed within 22 days; whereas, the surgical wounds of MSCDG rats were closed in 14 days. MSC infusion in dexamethasone-immunosuppressed patients contributed positively to epithelial healing in less time.

RESUMO:A anomalia de Pelger-Huët é um distúrbio hereditário raro caracterizado pela falha na segmentação do núcleo dos granulócitos, especialmente dos neutrófilos. Os neutrófilos geralmente apresentam núcleos hipossegmentados ou redondos, embora a condensação da cromatina e o desenvolvimento citoplasmático assemelham-se a de granulócitos maduros normais. A hipossegmentação do núcleo afeta 30 a 70% das células e mimetiza o estado de imaturidade celular, semelhante ao observado hematologicamente no desvio à esquerda. A anomalia de Pelger-Huët foi relatada em humanos, coelhos, cães e gatos, sendo esses relatos escassos e poucos casos foram descritos em animais e, no estado do Espírito Santo ainda não havia sido relatada. O reconhecimento dessa anomalia é importante para prevenir uma associação incorreta com doenças que produzem um desvio à esquerda. Relatamos um caso da anomalia de Pelger-Huët em uma cadela da raça Cocker Spaniel com 9 anos de idade. Palavras-chave: cão; desvio a esquerda; hematologia; hipossegmentação de neutrófilos PELGER-HUËT ANOMALY -CASE REPORTABSTRACT: Pelger-Huët anomaly is a rare hereditary disorder characterized by failure of the nucleus of granulocytes, especially neutrophils, to undergo normal maturation to the segmented form. Affected neutrophils have unsegmented or round nuclei, but chromatin condensation and cytoplasmic development resemble that of normal mature granulocytes. This nuclear hyposegmentation results in an apparent left shift in neutrophils. Affected animals have a normal total leukocyte count, but the major form of neutrophils is at the band stage, with bands comprising 30-70% of total cells. The Pelger-Huët anomaly has been reported in humans, rabbits, dogs, and cats. Few cases had been described in animals and, in the state of Espiríto Santo not yet had been reported. Recognition of this syndrome is important to prevent an incorrect association with diseases that produce a left shift. A case report of the Pelger-Huët anomaly is presented in a 9 years old Cocker Spaniel female dog.