Ce~ical lacerations Congerutal defects Dislocation of hip Prominen~head of ulna Bilateral shorterung of 5th finger Poiydactylism Dislocated wrist FlexIon deformity, fingers

In a previous communication from this laboratory (Dineen and Barter, a series of changes, including the presence of curious laminated cytoplasmio bodies, were described as occurring in tlie pancreatic acinar cells of adult mice infected with a local strain of Pleurodynia virus (designated as the PL5 strain by Atkinson, Dineen and Robertson, 1951). Because these changes seemed interestinfr, complex and somewhat unusual, and as it was not possible to make out many fine structural details with the visible-light micro.scope, a study was undertaken with the electron microscope in thin sections of the infected pancreas. METHODS.Preparation of virus suspensions. For inoculation of adult mice, a 20 p.c, muscle suspension waa prepared in fhilied physiological saline from the limbs of infant mice which were moribund from tho 19th pas.sagc of the PL5 virus. The crude suspension was spun in the "Spineo" ultracfntrifuge at 15,000 R.P.M. for 10 niinutea, aud the supernatant harvested. After the demonstration of bac^toriologipal sterility by culture, this preparation was inoculated intraperitoneally into adult mice in doses of 0*2 ml. A control 20 p.e. suspennion was prepared in an identical way from the muscles of non-Infected infant mice.Histological procedures. The methods used in this laboratory for preparing tissues for examination in the electron micro.seope have been described in a previous paper (Robertson, 1954). In addition, portions of each pancreas were taken into 10 p.e. formalin aniS after embedding in paraffin were stained with haematoxylin-cosin and haematoxylin-phloxine-tartrazine for examination in the visible-light microscope.Animals. 62 male albino mice, aged 6 to 8 weeks, from a stock colony were used. There were 40 infected animals, fi control animals injected with a non-infected 20 p.c. muscle suspension, and 10 normal controls. In addition, (i animals were used in a small preliminary experiment designed to exclude the possibility that the cellular changes seen were merely the result of the death of the cell with ensuing post-mortem degeneration. Animals were killed at intervals beginning at fi hours and ending at 12 days after inoculation. Two blocks from the pancreas of each animal, tixed in buffered 1 p.e. osmie acid solution, were cut and examined in the electron microscope. RESULTS.Naked-eye changes. 18 hours after intraperitoneal inoculation of the PL5 virus the adult mouse shows no alterations in appearance and beiiaviour, and on sacrifice the pancreas appears quite normal to the naked eye. At 24 hours, Auatral.

SUMMARY.In a controlled experiment using 86 pairs of male white mice matched for weight, the blood alcohol level after intraperitoneal injection of ethyl alcohol was slightly but significantly elevated in animals in which a dosed fracture of the femur was produced at the time of the alcohol injection. Significant elevation of blood alcohol was present at half an hour after alcohol injection and was still present three hours after injection. Blood alcohol levels in both injured and control animals decreased linearly at the same rate with the logarithm of time, but the regression lint-of the blood alcohol levels of the injured animals was higher than that of the control animals.

No abstract

Hince the well-known work of Sabin (1989) there has been increasing interest in the cytology of immunity. Using R-salt-azoprotein as the antigen, Sabin observed in fixed sections of rabbit lymph nodes and in cell suspensions appearances which she interpreted as representing the shedding of peripheral portions of the cytoplasm of the macrophages which had ingested the coloured antigen. She suggested that the shed fragments contained the antibody. Pollowing these earlier studies interest then shifted to the possible role of the lymphocyte