Purpose:To evaluate macro and microscopically the late evolution of autotransplants of fragments of spleen in the greater omentum, mesenterium and peritoneal cavity, after 24 weeks of observation. Methods: Fifty two Wistar rats were used, males and adults, submitted to total splenectomy and divided in four groups. The group I -seventeen animals with implant of spleen fragment in the peritoneal cavity; group II -eighteen animals with implant in the omentum and group III -seventeen animals with implant fixed in mesenterium root. The group control (group IV) was formed by eight animals chosen aleatorily among the three groups. It was analyzed macro and microscopically the evolution of the implant, and in the histological study qualitative and quantitative criteria were adopted, with the counting of no cellular e cellular elements. Results: It was observed adherences to the adjacent tissues and vascularization in all of the fragments transplanted. The group I presented white pulp and preserved vascularization. In the group II were observed white pulp with follicular formations and lymphoid tissue preserved, and the red pulp in cordon aspect and hemorrhagic. In the group III were observed with depletion of white and red pulp, while others evidenced better preservation of the pulps. The counting of lymphocytes revealed significant difference between the groups I and IV and the group III and IV (p < 0.05). The counting of active macrophages revealed significant difference between the groups II and III (p < 0.05) and similarity between II and IV (p > 0.05). The other elements: active macrophages phagocyting hemosiderine, plasmocytes, fibroblasts, fibrocytes, giant cells, monocytes, interstitial spaces and fibers of collagen, did not show significant difference among the groups. Conclusions: The splenic autotransplantation is feasible, being the better place the greater omentum. This research demonstrated through qualitative and quantitative histological analysis that the splenic tissue autotransplanted in the omentum of Wistar rats preserves its function of defense of the organisms. Key words: Spleen. Transplantation, Autologous. Macrophages. Rats. RESUMO Objetivo:Avaliar macro e microscopicamente a evolução tardia do autotransplante de fragmentos de baço no grande epiplon, mesentério e cavidade peritoneal, após 24 semanas de observação. Métodos: Foram utilizados 52 ratos Wistar, machos e adultos, submetidos a esplenectomia total e divididos em quatro grupos. O grupo I -dezessete animais com implante de fragmento de baço solto na cavidade peritoneal; grupo II -dezoito animais com implante no grande epiplon e grupo III -dezessete animais com implante fixado na raiz do mesentério. O grupo controle (grupo IV) foi formado por oito animais escolhidos aleatoriamente entre os três grupos. Foram analisados macro e microscopicamente a evolução do implante, sendo que no estudo histológico foram adotados critérios qualitativos e quantitativos, com a contagem de elementos celulares e não celulares. Resultados: Foram observ...

PURPOSE:To evaluate macro and microscopically the evolution of autotransplants of fragments of spleen different fragments in the greater omentum, after eight weeks of observation. METHODS:Twenty rats Wistar were used, males and adults, submitted to total splenectomy and divided in two groups. The group Iten animals with implant of spleen fragment (25% weight of spleen) in the omentum; and group II -ten animals with implant of spleen fragment (30% weight of spleen) in the omentum. It was analyzed macro and microscopically the evolution of the implant. RESULTS:It was observed adherences to the adjacent tissues and vascularization in all of the fragments transplanted. The group I and II presented white pulp with follicular formations and lymphoid tissue preserved, and the red pulp in cordon aspect. The group II presented white pulp more disorganized and red pulp hemorrhagic. The active macrophages were observed in the group I and II. CONCLUSION:The splenic autotransplantation of the group I showed better regeneration.Key words: Transplantation, Autologous. Spleen. Histology. Rats. RESUMO OBJETIVO:Avaliar macro e microscopicamente a evolução do autotransplante de diferentes fragmentos de baço no omento maior, após oito semanas de observação. MÉTODOS:Foram utilizados 20 ratos Wistar, machos e adultos, submetidos a esplenectomia total e distribuídos em dois grupos. O grupo I -dez animais com implante de fragmento com 25% do peso do baço no omento e o grupo II -dez animais com implante de fragmento com 30% do peso do baço no omento. Foram observados macro e microscopicamente a evolução dos implantes. RESULTADOS:Foi observada no fragmento transplantado aderência aos tecidos adjacentes e vascularização preservada. Os grupos I e II apresentaram polpa branca e vascularização preservada, polpa branca com formação folicular e tecido linfóide preservado, e a polpa vermelha com aspecto cordonal. O grupo II apresentou polpa branca mais desorganizada e polpa vermelha hemorrágica. Os macrófagos ativos foram observados nos grupos I e II. CONCLUSÃO:O autotransplante esplênico do grupo I mostrou melhor regeneração.Descritores: Transplante Autólogo. Baço. Histologia. Ratos. Histological aspects of autologous transplantation of different fragments of the spleen in rats

Background: Chronic superficial keratitis (CSK) is an ocular condition in dogs characterized by corneal opacification leading to visual function impairment. Control of this chronic condition requires use of topical immunomodulators or corticosteroids daily. Regenerative medicine has shown promising results in several fields of medicine. Aim: The aim of this study was to evaluate the clinical effect of allogeneic mesenchymal stem cells (MSCs) of adipose tissue applied via subconjunctival in dogs with CSK. Methods: A series of cases of 8 dogs diagnosed with CSK were divided into 2 groups, 4 dogs each, the conventional treatment (CT) received Prednisolone 1% as topical eye drops and the experimental group (EG) received allogeneic MSCs transplantation. The dogs had not previously been treated for CSK. Systemic and ophthalmologic examinations were performed to exclude other abnormalities. An administered amount of MSC (1x106 cells each time) were injected via subconjunctival in the peri-limbal region at 0 and 30 days. The animals were followed for 110 days to clinical evaluation, and, at the same time, the images of the corneal abnormalities were obtained and analyzed in the ImageJ software. The statistical analysis was performed in the GrandPrism 7.0 software. Results: Initial and final images revealed that areas with neovascularization, inflammatory infiltrate and opacity regressed in most eyes in both groups (7/8 eyes in each group) at the end of the 110 days, p = 0.0391 and p = 0.0078 respectively, but this response was minor in the EG comparing to CT (p = 0.026). No local or systemic side effects were observed. Conclusions: Despite the small melioration, MSCs treatment suggests clinical improvement in patients with CSK after 110 days without any local or systemic side effects. However, the improvement achieved was significantly less than the observed within CT group. Further studies still are needed to evaluate the use and benefits of stem cells as an adjunct treatment for CSK.

The application of BoNT/A into the levator palpebral superioris muscle in dogs was effective and safe to promote protective ptosis with a temporary covering of the cornea.