Goat ovaries were collected from the slaughterhouse and categorized as right, left, corpus luteum (CL)-present and -absent group and evaluated on the basis of weight (g), length (cm), width (cm), number of follicles, follicles aspirated and number and state of cumulus-oocyte-complexes (COCs). Comparatively higher weight [(0.66+/-0.02) vs (0.64+/-0.02) g], length [(1.17+/-0.02) vs (1.11+/-0.02) cm] and width [(0.77+/-0.02) vs (0.74+/-0.02) cm] were found in right ovaries than those of left. On the other hand significantly (P<0.05) higher weight [(0.71+/-0.03) vs (0.64+/-0.01) g] and width [(0.76+/-0.03) vs (0.75+/-0.01) cm] were found in CL-present group than those of CL-absent group of ovaries. The left ovaries contained comparatively higher number of normal COCs [(1.06+/-0.09) per ovary] than right ovaries [(1.03+/-0.10) per ovary] and the similar trend was found in total number of follicles [(4.51+/-0.25) vs (4.30+/-0.23) per ovary] and follicles aspirated [(2.55+/-0.14) vs (2.52+/-0.12) per ovary]. But the total COCs per ovary was almost similar in both ovaries [right and left: (1.85+/-0.12) and (1.85+/-0.11) per ovary, respectively]. Higher number of total COCs [(1.87+/-0.09) vs (1.76+/-0.16) per ovary], total number of follicles [(4.45+/-0.19) vs (4.16+/-0.37) per ovary], follicles aspirated [(2.55+/-0.10) vs (2.48+/-0.21) per ovary] and normal COCs [(1.12+/-0.07) vs (0.76+/-0.14) per ovary] were found in CL-absent group than those of CL-present group of ovaries.

A rapid new method for the simultaneous determination of tryptophan (Trp) and its related compounds such as indolelactic acid (ILA), tryptophol (TPP), tryptamine (TPM), indole-3acetaldehyde (ICA), indoleacetic acid (IAA), indole-3-aldehyde (ILD), skatole (SKT) and indole (IND), and p-hydroxyphenylacetic acid (HPA) and trans-cinnamic acid (CNM) which appeared within the retention time of final compound, SKT, in the chromatogram was established by highperformance liquid chromatography (HPLC). LiChrospher 100 RP-18 column and methanol-50 mM sodium acetate buffer (pH 4.2) (30: 70, v/v) as a mobile phase were used for the analysis. The compounds were monitored at 280nm with a UV absorbance detector. Prior to the analysis, sample was mixed with an equal volume of 4% (w/v) sulfosalicylic acid (SSA) for deproteinization. A 5μl portion of deproteinized and filtered sample was injected into the HPLC and the final peak of SKT appeared at about 60min. The recovery of every compound was more than 95%. The minimum detectable limits (pmol/5μl) of the secompounds were as follows: Trp, ILA, IAA,

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Rumen contents from three fistulated Japanese native goats fed Lucerne hay cubes (Medicago sativa) and concentrate mixture were collected to prepare the suspensions of mixed rumen bacteria (B), mixed protozoa (P) and a combination of the two (BP). Microbial suspensions were anaerobically incubated at 39 degrees C for 12 h with or without 1 mM of L-phenylalanine (Phe). Phe, tyrosine (Tyr) and other related compounds in both supernatant and microbial hydrolysates of the incubations were analyzed by HPLC. Tyr can be produced from Phe not only by rumen bacteria but also by rumen protozoa. The production of Tyr during 12 h incubation in B (183.6 mumol/g MN) was 4.3 times higher than that in P. One of the intermediate products between Phe and Tyr seems to be p-hydroxyphenylacetic acid. The rate of the net degradation of Phe incubation in B (76.0 mumol/g MN/h) was 2.4 times higher than in P. In the case of all rumen microorganisms, degraded Phe was mainly (> 53%) converted into phenylacetic acid. The production of benzoic acid was higher in P than in B suspensions. Small amount of phenylpyruvic acid was produced from Phe by both rumen bacteria and protozoa, but phenylpropionic acid and phenyllactic acid were produced only by rumen bacteria.