Qualitative and quantitative analysis of goat ovaries, follicles and oocytes in view of in vitro production of embryos

This study was conducted to evaluate the buffalo ovaries, follicles and cumulus-oocyte-complexes (COCs) with the view of in vitro production (IVP) of embryos. Buffalo (Bubalis bubalis) ovaries were collected from local slaughterhouse and categorized according to the position of ovaries in the genital tract (left vs. right) and presence or absence of corpus luteum (CL). Moreover, collected ovaries were also evaluated for length, width and weight, number of follicles, aspirated follicles and state of COCs. The length (cm) of right ovary (2.32±0.06) was significantly (P<0.05) higher than left ones (2.14±0.05). Number of follicles were higher (P<0.05) in the left (7.25±0.31) compare to the right (6.22±0.32) ovaries. Other parameters, including width, weight and number of COC aspirated did not differ significantly (P<0.05) between right and left ovaries. The length, width and weight of ovaries with CL were higher (P<0.05) whereas, number of observed follicles, aspirated follicles, number of COCs and number of normal COCs were significantly (P<0.05) higher in ovaries without CL than their counter parts.

Section: Resultssupporting

confidence: 88%

Section: Values In the Parentheses Indicate The Number Of Observationsupporting

confidence: 83%

Qualitative and quantitative analysis of buffalo ovaries, follicles and oocytes in view of in vitro production of embryos

Khandoker

Jahan

Asad

et al. 2012

“…Similar results also reported in goat (Asad, 2015;Saha et al, 2014;Mondalet al,2008 andIslam et al (2007). Those findings further supported the statement that for economic reason, typically less reproductive performing cows are slaughtered in slaughterhouse and most of them might be non- cyclic.…”

Section: Discussionsupporting

confidence: 84%

Effect of ovarian types and collection techniques on the number of follicles and the quality of cumulus-oocyte-complexes in cow

Mamy

Atiqah

Ariani

2017

This experiment was conducted for evaluation of bovine slaughterhouse ovary, follicles, and cumulus oocyte-complexes (COCs) and to compare COCs. The collected slaughterhouse ovaries was classified as corpus luteum present (CL+) and corpus luteum absent (CL-) groups. It was found that 62.5% of the ovaries collected were CL were CL+ type. For collection of COCs, blunt dissection and aspiration techniques were performed and number of follicles collected was recorded. The higher number of follicles were dissected and aspirated from CL -ovaries (11.2±1.8and 37.8±14.9, 28.3±15.6, respectively). The follicular materials collected from both techniques were observed under microscope to categorize the COCs as A (oocyte surrounded with cumulous cells homogenously), B (oocyte surrounded with cumulous cells partially), C (oocyte not surrounded at all by cumulous cells) and D (degeneration observed both in oocyte and cumulous cells). Grade A and grade B were classified as normal and grade C and grade D were considered as abnorma ovaries having no CL contributing more total number of COCs per ovary (6.8±1.0) and also contributing higher normal COCs (5.7±0.9) than that of ovaries with CL (6.0±2.0 and 4.5±1.5, respectively) in blunt dissection technique. But same trend of result was not found in aspiration technique. Similarly, higher percentage of COCs recovery rate was also recorded in blunt dissection (61.6±4.6% vs 16.5±4.9%, on total basis) than aspiration (48.6±2.9% vs 11.7±4.1%, on normal bas without CL and blunt dissection technique found more suitable for harvesting the higher number and superior quality of COCs for extending the

“…However, in our country this area is still untouched. In Bangladesh context, in vitro production (IVP) of embryos has been practiced 7 in different species such as mouse (Khandoker et al 2005); cattle (Rahman et al 2003;Goswami et al 2004 andPervage 2007), goat (Ferdous 2006;Islam et al 2007 andMondal et al 2008) and buffalo (Jahan 2009). However, IVP of interspecies hybrid embryos is not performed in our country yet.…”

Section: Introductionmentioning

confidence: 99%

In vitro maturation of buffalo oocytes and fertilization by cattle spermatozoa

Khandoker

Reza

Asad

et al. 2012

The present research was undertaken to explore the maturation of buffalo oocytes using bovine follicular fluid (BFF) and bovine serum albumin (BSA), as well as subsequent fertilization using cattle spermatozoa. The cumulus oocyte complexes (COCs) were collected by aspiration of slaughterhouse buffalo ovaries. Maturation was performed in TCM 199 supplemented with 10% BFF, 5% BSA or without supplementation (control). The COCs were fertilized in Brackett and Oliphant (BO) medium using capacitated fresh cattle spermatozoa. It was observed that the percentage of COCs reached to M-II stages were 40.78±3.84, 65.74±2.39 and 67.52±0.85; normal fertilization (formation of 2 pronuclei) were 23.28±3.00, 29.30±0.73 and 30.52±1.21 for control, 10% BFF and 5% BSA supplementation, respectively. The supplementation of BFF (10%) and BSA (5%) were given similar results on maturation and increased significantly (p<0.05) than that of the control. It was observed that cattle spermatozoa were fertilized by the buffalo oocytes and the fertilization rate was 23.28% to 30.52% in BFA and BSA supplemented media, respectively. It can be concluded that buffalo oocytes might be fertilized using capacitated cattle spermatozoa and both 10% BFF and 5% BSA could be supplemented in maturation media to enhance the maturation rate as well as fertilization of buffalo oocytes.