Effect of ovarian types and collection techniques on the number of follicles and the quality of cumulus-oocyte-complexes in cow

Abstract

This experiment was conducted for evaluation of bovine slaughterhouse ovary, follicles, and cumulus oocyte-complexes (COCs) and to compare COCs. The collected slaughterhouse ovaries was classified as corpus luteum present (CL+) and corpus luteum absent (CL-) groups. It was found that 62.5% of the ovaries collected were CL were CL+ type. For collection of COCs, blunt dissection and aspiration techniques were performed and number of follicles collected was recorded. The higher number of follicles were dissected and aspirated from CL -ovaries (11.2±1.8and 37.8±14.9, 28.3±15.6, respectively). The follicular materials collected from both techniques were observed under microscope to categorize the COCs as A (oocyte surrounded with cumulous cells homogenously), B (oocyte surrounded with cumulous cells partially), C (oocyte not surrounded at all by cumulous cells) and D (degeneration observed both in oocyte and cumulous cells). Grade A and grade B were classified as normal and grade C and grade D were considered as abnorma ovaries having no CL contributing more total number of COCs per ovary (6.8±1.0) and also contributing higher normal COCs (5.7±0.9) than that of ovaries with CL (6.0±2.0 and 4.5±1.5, respectively) in blunt dissection technique. But same trend of result was not found in aspiration technique. Similarly, higher percentage of COCs recovery rate was also recorded in blunt dissection (61.6±4.6% vs 16.5±4.9%, on total basis) than aspiration (48.6±2.9% vs 11.7±4.1%, on normal bas without CL and blunt dissection technique found more suitable for harvesting the higher number and superior quality of COCs for extending the

Evaluation of Different Techniques in Recovering of Oocytes and Storage Duration of Ovaries on the Quality and Quantity of Bovine in Vitro Maturation

“…The collection technique of oocytes was no significant effect (P>0.05) on oocytes quality. Similar result and same trend was reported by Mamy et al (2016) that higher percentage of COCs recovery was also recorded in blunt dissection (61.6±4.6%) than aspiration (48.6±2.9%) technique. This result lower than that of Singh et al (2019) who reported the recovery of A+B grade oocyte was 86.01% and 81.76% by aspiration and slicing technique, respectively.…”

“…This result supported by Habeed and Hussain (2017) who reported that the slicing method yielded significantly higher number of oocytes, and Wang et al (2007) found that when compared with many collection methods of bovine oocytes, the slicing method yield more oocytes count than the other methods. Result of this study was higher than that of Mamy et al (2016) was reported 10.5±1.5 in ovary with CL and 11.2±1.8 in ovary without CL. Nandi et al (2000) stated that when ovaries had a corpus luteum, the oocytes recovery rate decreases.…”

Seleção e classificação de oócitos bovinos post mortem

“…Um número indeterminado de ovários coletados apresentava presença de corpo lúteo. Batista (2016) afirma que no ponto de vista morfológico, a presença de corpo lúteo não tem influência na qualidade dos oócitos imaturos, já Mamy et al (2017) afirma que a presença de corpo lúteo em ovários aspirados pode resultar em uma quantidade maior de oócitos inviáveis comparados a ovários que não apresentavam, assim tendo mais oócitos viáveis aspirados.…”