The purpose of this study was to know the quality of Boer liquid semen during storage by adding sweet orange essential oil and gentamicin into the tris yolkextender.The semenlongevity test was carried out by storing semen in a closed tube at room temperature and refrigerator, and evaluated motility and Viability every 3 hours at room temperature and 12 hours in refrigerator. The results showed that the characteristics of the liquid semen, with the addition of 1% of essential oils showed percentage of motility and Viability significantly higher than 0.5% and without the addition of essential oil, either stored at room temperature or in the refrigerator. This may be related to the content of essential oils of sweet orange peel containing flavonoids and antibacterials that are capable of maintaining liquid semen quality of Boer Goat.In addition, gentamicin contains antibacterials capable of suppressing the growth of bacteria which can damage and durabilitythe spermatozoa.Keywords: Boer Goat,Gentamicin, Liquid semen, Sweet orange

This study aimed to evaluate different recovery techniques of oocytes and the duration time between ovary collection and processing in the laboratory on the quality and quantity of Simmental cross bovine oocytes in vitro maturation. A total of 75 bovine ovaries were divided into three groups. Experiment-1;  the storage duration of ovaries were 6, 9 and 12 h. Experiment -2;  the techniques for oocytes recovery were slicing, aspiration and slicing + aspiration. The ovaries were collected from slaughterhouse and kept in saline solution during transportation to laboratory. A total of 136 oocytes were used for experiment-1, and 246 oocytes for experiment-2. This study found that the number and quality of oocytes were significantly different (P<0.05) as the effect of storage time. The average number of oocytes were 15.90±2.48; 17.10±1.38 and 13.60±3.00, in 6 h; 9 h and 12 h of storage time, respectively. The techniques of oocytes collection significantly (P<0.05) affect the quantity of oocytes but no significant (P>0.05) on oocytes quality. The average of oocytes of groups slicing, aspiration and slicing plus aspiration were 14.66±2.09; 9.46±2.99 and 16.40±6.86, respectively. Both experiment was significant effect (P<0.05) on immaturation rate of bovine. Conclusion of this study the storage time of ovaries was suitable in 9 h and the technique of oocytes collection was in slicing plus aspiration.

This paper aims to find out the application of I.B. in cows and the control of estrus to determine fertility and conception rate in cows. The research was conducted in two stages: field research to determine the influence of I.B. time on conception rate. Local cows in I.B. amounted to 62 heads, and crossbred cows with as many as 100 heads were grouped based on I.B. time and parity and analysed with the Z test. Fertility is determined by the size of the dominant follicle using ultrasonography, and the number of gardenings is determined 30 days after I.B. and 60 days of rectal palpation. Average conception rate at the beginning of estrus (45.70%), middle estrus (60.59%), end of estrus (68.91%), and six hours after estrus (61.55%). I.B. time had a significant effect (P<0.05) on the conception rate. Based on the results of this study, it was revealed that parity-3 had the highest pregnancy rate for local cattle (70.34%), while crossbreed cattle had the highest pregnancy rate at parity-2 (56.79%). The follicle size in Bali cattle was the same as that of local south coast cattle, including the small follicle size category.

Tujuan dari penelitian ini adalah untuk mengamati kualitas spermatozoa epididimis kambing kacang  yang di preservasi pada suhu 5°C. Spermatozoa dikumpulkan dari bagian cauda epididimis dengan kombinasi metode slicing dan penekanan. Parameter yang diamati adalah persentase motilitas progresif sperma hidup dan abnormalitas. Hasil penelitian menunjukkan bahwa persentase motilitas progresif semen cauda epididimis setelah preservasi 0, 1, 2 dan 3 jam adalah  tidak pengaruh nyata (P>0,05). Selanjutnya persentase hidup berpengaruh sangat nyata (P<0,01) dengan nilai rataan perlakkuan tertinggi didapat pada lama simpan ke 0 jam yaitu 66,875±19,49%. Untuk persentase abnormalitas tidak memberikan pengaruh yang nyata (P>0,05). Dapat disimpulkan, bahwa pengaruh lama preservasi semen cauda epididimis yang berbeda pada suhu 5˚C tidak berpengaruh nyata (P>0,05) terhadap persentase motilitas dan persentase abnormalitas, dan berpengaruh sangat nyata (P<0,01) terhadap persentase hidup.

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