The genome groups of 34 wild and cultivated banana samples collected from 9 districts of Manipur, India were analyzed using IRAP and RAPD. IRAP-PCR analysis of the banana genome revealed that 32 out of 34 banana samples have shown presence of multiple polymorphic bands in amplified products. There were 5 genome specific bands (2 for B genome and 3 for A genome) in all the samples analyzed by using IRAP marker. Analysis of A and B genome among plant samples using RAPD-PCR generated a total of 1425 polymorphic bands by six primers (OPF-6, OPX-6, OPK-12, RAPD-6, RAPD-14 and RAPD-17). The highest number of bands was generated by OPK12 (318) followed by RAPD6 (308 bands), OPX6 (263 bands), RAPD 17 (215 bands), RAPD 14 (165 bands) and OPF6 (156 bands) respectively. Among these primers, RAPD 14 displayed specific bands per sample thereby showing higher delineating power than other primers.
Influence of urea and farmyard manure compost as N source on symbiotic association between Rhizobium leguminosarum (MTTC 10096)-Neptunia prostrata (Lam.) Baill Abstract Growth, biomass production, nitrogen fixation and nodulation in Neptunia prostrata (Lam.) Baill. is affected differently by various N sources and concentrations but the subject needs to be discussed in more depth. Effects of urea and farmyard manure compost (FYMC) as N source on the symbiotic association between Rhizobium leguminosarum MTTC10096-Neptunia prostrata (Lam.) Baill., biomass production, nitrogen accumulation, etc., at three different concentrations in loamy soil culture were investigated. The total N in both urea and FYMC were of equal proportion, but FYMC was more beneficial in overall performance of Neptunia prostrata plant. Nodule number and biomass were greatly depressed but plant length and weight showed slight increase by the higher N concentration in all the samplings. The study revealed that higher concentration of urea has more negative effect on nodule biomass and number than FYMC as compared to control. The plants also showed better N percent accumulation on the application of FYMC than urea. The overall performance of the plant was more pronounced on FYMC application as N source. It might be due to the presence of other vital nutrients in FYMC other than N, essential for both the host plant and microsymbiont. This aspect, however, awaits further experimental proof.
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