Microbially-induced calcite precipitation (MICP) is an emerging ground-modification technique. This paper presents the results of laboratory experiments that elucidate some biological factors affecting bioaugmentation and biostimulation strategies of MICP. Co-culture experiments suggest that ureolytic bacterium Sporosarcina pasteurii (DSMZ 33) might release the enzyme urease once introduced into a medium containing non-ureolytic bacterium Bacillus subtilis (DSMZ 6397) due to lysis by the latter, resulting in uncontrolled calcite precipitation. This suggests that exogenous bacteria introduced into a native soil might not survive due to adverse action by indigenous bacteria. It is shown that effective biostimulation of indigenous ureolytic bacteria in low-nutrient sand can be achieved using a stimulation medium containing 200 mM urea, complemented with a simple carbon source (molasses). Changes in microbial population following stimulation were quantified, using genetic enumeration, to show that (a) the net increase in urease activity is not accompanied by increases in the relative abundance of ureolytic bacteria, (b) nitrifying bacteria are part of the enriched indigenous population and (c) nitrifying bacteria can be stimulated by the addition of ammonium only. The use of the lowest effective urea concentration and simple carbon is advocated for sustainable biostimulated MICP, yielding lower ammonium emissions and reduced post-treatment recovery overheads.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.