Wild rice is an important reservoir of valuable and useful genes. O. rufipogon and O. nivara contain AA genome andare the progenitor of cultivated rice which makes them compatible with the cultivated rice for cross breeding to incorporate the genes for stress tolerance. SSR markers were used to assess the extent of diversity of 26 accessions of O. rufipogon and O. nivara collected from different districts of Orissa, West Bengal and Tripura. The Principal Co-ordinate Analysis (PCA) clearly indicates the clustering pattern and inter-relationships among different accessions. Mantel Z-testexhibitedacorrelation between cophenetic matrix and Jaccards’ similarity coefficient in 26 accessions and 4 CRRI released varieties using 54 STMS (SSR) markers which showed significant correlation (r = 0.8249) between them. O. nivara accessions and O. rufipogon accessions were grouped different clusters. O. nivara collected from Midnapore is placed in different cluster. It is concluded that the SSR markers used were found to be equally informative for the genetic diversity study between and among the accessions of two wild species such as O. rufipogon and O. nivara collected from different locations of Orissa, West Bengal & Tripura. Highlysignificant morphological variations were also observed among O. nivara and O. rufipogon accessions.

A b s t r a c t A r t i c l e I n f oThe variation in total proline content in six greengram cultivars i.e., 24 ML-233 (R), 7 GGG 10-14 (R), 17 IPM 9901-6 (R), 8 GM 04-02 (R), 28 PM 10-12(S) and 29 PUSA 0672 (S) were studied 45 days after inoculation of Meloidogyne incognita. The total proline content in the shoots of both healthy and resistant cultivars was higher compared to the susceptible cultivars. However, in the inoculated plants the total proline content in four resistant variety varied from 100 mg/g to 150 mg/g as compared to two susceptible variety (90 mg/g to 140 mg/g) . An increasing trend was also observed in the proline contents in the shoots of inoculated susceptible and resistant cultivars.

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