Cancer prevention by dietary phytochemicals has been shown to involve decreased cell proliferation and cell cycle arrest. However, there is limited understanding of the mechanisms involved. Previously, we have shown that a common effect of phytochemicals investigated is to oxidize the intracellular glutathione (GSH) pool. Therefore, the objective of this study was to evaluate whether changes in the glutathione redox potential in response to dietary phytochemicals was related to their induction of cell cycle arrest. Human colon carcinoma (HT29) cells were treated with benzyl isothiocyanate (BIT), diallyl disulfide (DADS), dimethyl fumarate (DMF), lycopene (LYC), sodium butyrate (NaB) or buthione sulfoxamine (BSO, a GSH synthesis inhibitor) at concentrations shown to cause oxidation of the GSH: glutathione disulfide pool. A decrease in cell proliferation, as measured by [ 3 H]-thymidine incorporation, was observed that could be reversed by pretreatment with the GSH precursor and antioxidant N-acetylcysteine (NAC). Cell cycle analysis on cells isolated 16 h after treatment indicated an increase in the percentage (ranging from 75% to 30% for benzyl isothiocyanate and lycopene, respectively) of cells at G2/M arrest compared to control treatments (dimethylsulfoxide) in response to phytochemical concentrations that oxidized the GSH pool. Pretreatment for 6 h with N-acetylcysteine (NAC) resulted in a partial reversal of the G2/M arrest. As expected the GSH oxidation from these phytochemical treatments was reversible by NAC. That both cell proliferation and G2/M arrest, were also reversed by NAC leads to the conclusion that these phytochemical effects are also mediated, in part, by intracellular oxidation. Thus, one potential mechanism for cancer prevention by dietary phytochemicals is inhibition of the growth of cancer cells through modulation of their intracellular redox environment.

No abstract

No abstract

Big Data Infrastructure at the Crossroads 3 perceptions that much data is either derivative, low quality, or gathered from sources that are inappropriate for open sharing. ▪ Ethical Challenges. The ethical dimensions of big data research remain contested, and some researchers are uncertain about best practices for ethical research conduct. Although IRB guidance is valued, some researchers expressed concerns that IRB regulations are not well adapted to new or evolving research methods. ▪ Support and Training. Researchers tend to favor informal training methods, such as internet tutorials, over formal training in big data methods. While such methods work well for solving immediate problems, they are less well suited to acquiring foundational knowledge, leaving the potential for blind spots in academic research.

This chapter describes a new and emerging type of research and writing support for junior faculty members. The Academic Writing Institute was born of a collaboration between academic librarians and tenured faculty at the campuses the library supports. Grant funded, the purpose of the academic writing institute was to: 1) provide junior faculty with an overview of the services offered by the library that can help to facilitate their research processes; 2) introduce methods for documenting research findings in the 21st century publishing environment; and 3) provide a forum, led by tenured faculty, to enhance participant knowledge about the publishing processes associated with their specific disciplines. This new paradigm allows for other academic librarians to explore new methods by which the campus library can support faculty members with the research and publishing components of their promotion and tenure requirements.