The purpose of this study is to determine the quality of epididymis spermatozoa of buffalo after preservation with TALP media by adding serum with different level. The method used in this study is the experimental method and the design used is a randomized block design (RBD) with 3 treatment by adding bovine serum (0%, 5%, and 10%) and there are 6 replications as a group (repetition in the form of days). The result showed that the addition of serum into TALP diluent had a significant effect (P>0, 05) on the percentage of the motility of cauda epididymis spermatozoa of swamp buffalo after preservation which was 73, 33 in P2 10% serum and the viability is 79,167 ± 2,04 on P2 10%. This can be used as an indicator that the serum is effective in protecting spermatozoa from damages during the cryopreservation process, so by that, it would improve the quality of cauda epididymal spermatozoa of swamp buffalo. While the percentages of abnormality of cauda epididymis spermatozoa of swamp buffalo after preservation did not have a significant effect (P>0, 05). Based on the result of this study it can be concluded that the addition of 5% serum and 10% addition of egg yolk and 5% addition of glycerol will improve the quality of motility and viability and had no adverse effect on abnormalities after the preservation process at low temperature.

This research aimed to know the effect of the addition of jamblang leaves extract (Syzygium cumini) in skim milk-egg yolk diluent material on motility, abnormality, and viability of Aceh cattle stored at 4ºC. The research design used was Complete Randomized Design consisting of 5 treatment and 5 repetitions. The treatments consisted of J0 (SSKT), J1 (SSKT+ EDJ 0. g/ 100 ml), J2 (SSKT+ EDJ 0.4 g/ 100 ml), J3 (SSKT+ EDJ 0.6 g/ 100 ml) and J4 (SSKT+ EDJ 0.8 g/ 100 ml). The data obtained were analyzed descriptively by determining the mean value and standard deviation using Microsoft Excel. The research result indicated that the addition of jamblang leaves extract in skim milk-egg yolk diluent material maintaining the motility, viability and abnormality of the spermatozoa of Aceh cattle stored at the temperature of 4ºC. Based on the research that has been carried out,  it can be concluded that the addition of jamblang leaves extract in skim milk-egg yolk diluent material can maintain the quality of Aceh cattle spermatozoa, while the use of incorrect dose becomes toxic and disrupt the spermatozoa activities to cause death.