Aim: The aim of this study was to assess the barriers of research utilization among nurses of public hospitals Lahore, Pakistan. Methodology: Descriptive cross sectional study was used. This study was conducted among nurses of Mayo Hospital Lahore and Lady Aitchison Hospital Lahore. The data was collected through simple random sampling from 245 registered nurses from both hospitals. The questionnaire of barriers to research utilization developed by Funk et al. (1993) was used. Result: The current study results show that nurses didn't utilize the research in practices. Non-supportive organization, colleagues, inadequate facilities, insufficient time, nurse feels she has no authority to change patient care procedure, research factor and nursing factor is the main barrier of research utilization. In the future nurses should come on a paradigm of research. Conclusion: This reveals that management should increase the focus on nurse's knowledge and facilities about research utilization