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An effective method for decolorization of the polysaccharides from Lethariella spp. was studied. Decolorization rate was used as an indicator in the experiment, single factor text and orthogonal experiment was used to get the optimal conditions, the single factor experiment was carried out with temperature at 35°C, 45°C, 55°C, 65°C, and 75°C, the activated carbon added at 0.5%, 1%, 1.5%, 2%, and 2.5%, and time at 10, 15, 20, 25, and 30min, respectively. The optimal conditions of Lethariella spp. clarification using activated carbon were carried out to get the optimal conditions (the temperature at 55°C, the activated carbon added at 1%, and time was 25 min, and the decolorization rate could reach 57.56 %).

In order to survey the stability of carotenoids in Russula alutacea extraction. The survival rate of total carotenoid content in extract was determined under different conditions, including different light, temperature, metal ions, different pH solution, oxidizing and reducing agents. The results showed that all of these conditions could affect the stability of carotenoids in the extraction. The carotenoids were sensitive to sunlight and degraded rapidly in direct sunlight. The carotenoids had a greater loss with the increasing temperature. Acid environment may cause the carotenoids loss, but the carotenoids were relatively stable under alkaline environment. While zinc and ferric ions (Zn2+, Fe2+ and Fe3+), especially Fe3+, could obviously destroy carotenoids. In all, the reducing agent had certain protective effect on the stability of carotenoids. So when using or saving the carotenoids, it was necessary to be dark, low temperature and neutral condition, and avoided using copper and ferric ware. The results will provide a scientific basis for the development and utilization of carotenoids from R. alutacea in Yunnan.