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This study used Green Spinach Leaf Extract for the green synthesis (monocolors) of silver nanoparticles (AgNPs). This search focuses on the optimization of synthesis by evaluating the impact on the each of the size distribution and activity of antibacterial against bacteria (Escherichia coli) of the green spinach leaf extract volume percent. The characterization of AgNP's was carried out utilizing UV-Visible spectrophotometers, size examination of particles, transmission electron microscopes, energy spectrometry with dispersion X rays, Fourier infrared spectrometer transform and x-ray scanning methods. The experimental data show that AgNPs have been produced effectively and size of particle is controlled by the amount of green spinach leaf extract. With the size reaching (5 nm, polydispersity indices = 0.063 nm), the smaller volume percentage creates AgNPswith a spherically formed reach of 20%. The synthesized AgNPs by the spinach leaf extract had a good antibacterial activity especially at the concentration of (500μg/ml) in which the inhibition zone reached 35mm against gram negative bacteria Escherichia coli.