Neonatal necrotizing enterocolitis rat model attenuated by a remote ischemic preconditioning in the pregnant 1Acta Cir. Bras. 2017;32(3):236-242 Abstract Purpose: To evaluate the effect of remote ischemic preconditioning (r-IPC) administered to pregnant rats, in the ileum of newborn rats subjected to hypoxia and reoxygenation. Methods: We used three pregnant rats and their newborn rats distributed in three groups: 1) Control (C) -Newborn rats born from a pregnant rat which did not undergo any intervention; 2) Hypoxia-Reoxygenation (H/R) -Newborn rats born from a pregnant rat which did not undergo any intervention, and were subjected to hypoxia-reoxygenation; 3) Remote Ischemic Preconditioning (r-IPC) -newborn rats born from a pregnant rat which was subjected to remote ischemic preconditioning twenty-four hours before giving birth and the newborn rats were subjected to hypoxia-reoxygenation. Segments of ileum were prepared for histological analysis by HE and immunohistochemistry by the Ki67 to evaluate cell proliferation, crypt depth and villus height and evaluation of apoptosis by cleaved caspase-3. Results:The intensity of the lesions was lower in the r-IPC than in the H/R group, showing significant difference (p<0.01). The r-IPC group showed a higher proliferative activity compared to the H/R group (p<0.01), with deeper crypts (r-IPC > H/R -p < 0.05), and higher villi, showing significant difference (r-IPC > H/R -(p <0.01). The occurrence of apoptosis in the H/R group was lower in comparison to groups C and r-IPC, with significant difference (H/R < r-IPC; p<0.05). Conclusion:The remote ischemic preconditioning applied to the pregnant rat protected the ileum of newborn rats subjected to hypoxia and reoxygenation, with decreased intensity of the lesions in the ileum mucosa and preservation of proliferative activity, keeping the villus height and crypt depth similar to group C. Key words: Enterocolitis, Necrotizing. Ischemic Preconditioning. Pregnancy, Animal. Rats. The characteristic of r-IPC is the occurrence of two distinct phases of protection: early, which begins immediately after reperfusion, having protective action in a period of two to three hours, and the late one, which manifests itself between twelve and twenty-four hours after the initial reperfusion, acting for up to 72 hours 6,12 . 8-Experimental SurgeryIn our lab, it was shown that r-IPC applied on pregnant rat minimized the occurrence of colonic NEC in their pups 13 . Considering the systemic effect of r-IPC against IR lesions and that ischemia is a relevant factor in the occurrence of NEC; further, the ileum is also compromised in this disease; it was decided to test the hypothesis that r-IPC applied in pregnant rat might attenuate the small bowel lesions in an experimental NEC model. ■ MethodsThe experiments were conducted after approval by the Research Ethics Committee, Universidade Federal de São Paulo, under protocol no. CEP 0341/07.It was used three pregnant rats and their 31 newborns from the OUTBRED EPM-1 Wistar strain (Rattus norvegicus ...

PURPOSE:To evaluate the effects of maternal remote ischemic preconditioning (IPCr) in the colonic mucosa of newborn rats subjected to hypoxia and reoxygenation. METHODS:Newborn Wistar rats were divided into three groups. Control Group (CG), Hypoxia and Reoxygenation Group (HRG) and Remote Ischemic Preconditioning Group (IPCrG). Hypoxia and reoxygenation was performed 2x per day, with an interval of 6 hours, on the 1st, 2nd and 3rd days of life, with 10 minutes of CO 2 at 100%, followed by 10 minutes O 2 at 100%(HRG/IPCrG). The maternal IPCr was performed 24 hours before delivery by applying a rubber band tourniquet to the left hind limb (IPCrG). Segments of the colon underwent histological (HE) and immunohistochemical analysis for caspase-3 and COX -2. RESULTS:The histological findings showed no intestinal mucosal damage in the CG group and severe lesions in HRG that was attenuated in the IPCrG (p<0.05). The expression of the apoptotic cells was lower in the HRG group than in the CG and IPCrG. The COX-2 expression was intense in HRG and attenuated in the IPCrG (p<0.05). CONCLUSIONS:Maternal IPCr protected the colonic mucosa of newborn rats subjected to hypoxia and reoxygenation, reducing the morphological alterations and inflammatory response. It ameliorates the occurrence of apoptosis, keeping the physiological process of renewal and regeneration in the epithelial lining of the colonic mucosa.Key words: Enterocolitis, Necrotizing. Colon. Apoptosis. Rats. Effects of maternal ischemic preconditioning in the colon of newborn rats submitted to hypoxia-reoxygenation insultActa

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