Effects of maternal ischemic preconditioning in the colon of newborn rats submitted to hypoxia-reoxygenation insult

Freitas, Maria Andréia Lopes de; Gomes, Rúdnei de Oliveira Luciano; Soares, Bruno Leonardo de Freitas; Neto, Ricardo Artigiani; Montero, Edna Frasson de Souza; Martins, José Luiz

doi:10.1590/s0102-86502014000700005

Cited by 3 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…the Hypoxia/Reoxygenation group (Median of positive cells C=14 (10-15); H/R=8 (0-12); rIPC=12 (11)(12)(13)(14); r-IPCvsH/R, p=0.0002), which, in addition, showed decreased crypt depth and villus height. The remote ischemic preconditioning preserved the proliferative activity, as well the depth of the crypts (C=29 µm; H/R=16 µm; rIPC=24 µm, r-IPCvsH/R, p=0.01) and villus height (C=87 µm, H/R=45 µm; r-IPC=84 µm, r-IPCvsH/R, p=0.01) ( Figure 2).…”

Section: ■ Resultsmentioning

confidence: 98%

“…In our lab, it was shown that r-IPC applied on pregnant rat minimized the occurrence of colonic NEC in their pups 13 . Considering the systemic effect of r-IPC against IR lesions and that ischemia is a relevant factor in the occurrence of NEC; further, the ileum is also compromised in this disease; it was decided to test the hypothesis that r-IPC applied in pregnant rat might attenuate the small bowel lesions in an experimental NEC model.…”

Section: -Experimental Surgerymentioning

confidence: 93%

See 1 more Smart Citation

Neonatal necrotizing enterocolitis rat model attenuated by a remote ischemic preconditioning in the pregnant

et al. 2017

Self Cite

View full text Add to dashboard Cite

Neonatal necrotizing enterocolitis rat model attenuated by a remote ischemic preconditioning in the pregnant 1Acta Cir. Bras. 2017;32(3):236-242 Abstract Purpose: To evaluate the effect of remote ischemic preconditioning (r-IPC) administered to pregnant rats, in the ileum of newborn rats subjected to hypoxia and reoxygenation. Methods: We used three pregnant rats and their newborn rats distributed in three groups: 1) Control (C) -Newborn rats born from a pregnant rat which did not undergo any intervention; 2) Hypoxia-Reoxygenation (H/R) -Newborn rats born from a pregnant rat which did not undergo any intervention, and were subjected to hypoxia-reoxygenation; 3) Remote Ischemic Preconditioning (r-IPC) -newborn rats born from a pregnant rat which was subjected to remote ischemic preconditioning twenty-four hours before giving birth and the newborn rats were subjected to hypoxia-reoxygenation. Segments of ileum were prepared for histological analysis by HE and immunohistochemistry by the Ki67 to evaluate cell proliferation, crypt depth and villus height and evaluation of apoptosis by cleaved caspase-3. Results:The intensity of the lesions was lower in the r-IPC than in the H/R group, showing significant difference (p<0.01). The r-IPC group showed a higher proliferative activity compared to the H/R group (p<0.01), with deeper crypts (r-IPC > H/R -p < 0.05), and higher villi, showing significant difference (r-IPC > H/R -(p <0.01). The occurrence of apoptosis in the H/R group was lower in comparison to groups C and r-IPC, with significant difference (H/R < r-IPC; p<0.05). Conclusion:The remote ischemic preconditioning applied to the pregnant rat protected the ileum of newborn rats subjected to hypoxia and reoxygenation, with decreased intensity of the lesions in the ileum mucosa and preservation of proliferative activity, keeping the villus height and crypt depth similar to group C. Key words: Enterocolitis, Necrotizing. Ischemic Preconditioning. Pregnancy, Animal. Rats. The characteristic of r-IPC is the occurrence of two distinct phases of protection: early, which begins immediately after reperfusion, having protective action in a period of two to three hours, and the late one, which manifests itself between twelve and twenty-four hours after the initial reperfusion, acting for up to 72 hours 6,12 . 8-Experimental SurgeryIn our lab, it was shown that r-IPC applied on pregnant rat minimized the occurrence of colonic NEC in their pups 13 . Considering the systemic effect of r-IPC against IR lesions and that ischemia is a relevant factor in the occurrence of NEC; further, the ileum is also compromised in this disease; it was decided to test the hypothesis that r-IPC applied in pregnant rat might attenuate the small bowel lesions in an experimental NEC model. ■ MethodsThe experiments were conducted after approval by the Research Ethics Committee, Universidade Federal de São Paulo, under protocol no. CEP 0341/07.It was used three pregnant rats and their 31 newborns from the OUTBRED EPM-1 Wistar strain (Rattus norvegicus ...

show abstract

Section: ■ Resultsmentioning

confidence: 98%

Section: -Experimental Surgerymentioning

confidence: 93%

Neonatal necrotizing enterocolitis rat model attenuated by a remote ischemic preconditioning in the pregnant

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…The H-score was calculated by combining the percentage of immunoreactive cells (%) with an estimate of the staining intensity (0, 1, 2 or 3), as described previously (24). An H-score of 9-12 was considered to represent strong immunoreactivity ('+++'), 5-8 was considered moderate ('++'), 1-4 was considered weak ('+') and 0 was scored as negative ('-') (25,26).…”

Section: Culture Of Bmscs and Ucmscs Bmscs And Ucmscsmentioning

confidence: 99%

Comparative evaluation of the osteogenic capacity of human mesenchymal stem cells from bone marrow and umbilical cord tissue

Wang¹,

Zhao²,

Xing³

et al. 2018

Exp Ther Med

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) have been extensively investigated in the field of regenerative medicine. Human bone MSCs (BMSCs) have become a common type of seed cell for bone tissue engineering. However, the viability and cell number of BMSCs are negatively correlated with donor age, and as the extraction process is painful, this method has not been widely used. As human umbilical cord MSCs (UCMSCs) may be harvested inexpensively and inexhaustibly, the present study evaluated and compared the regenerative potential of UCMSCs and BMSCs to determine whether UCMSCs may be used as a novel cell type for bone regeneration. In the present study, the proliferation and osteogenic capacity of BMSCs and UCMSCs was compared in vitro. BMSCs and UCMSCs were respectively combined with biofunctionalized macroporous calcium phosphate cement, and their bone regenerative potentials were determined by investigating their capacity for ectopic bone formation in a nude mouse model as well as their efficacy in a rat model of tibia bone defect. The extent of bone regeneration was examined by X-ray, histological and immunohistochemical analyses. The results revealed that UCMSCs exhibited a good osteogenic differentiation potential, similarly to that of BMSCs, and that UCMSCs were able to contribute to the regeneration of bone and blood vessels. Furthermore, no significant differences were identified between BMSCs and UCMSCs in terms of their bone regenerative effect.

show abstract

“…In the small intestine dysfunction observed after sustained warm ischemia was prevented if preconditioning was carried out before the ischemia 4 . The mechanism of remote preconditioning is not limited to one organ system either: Freitas et al 5 noted that maternal IPC protected the colonic mucosa of neonatal rats subjected to hypoxia and reoxygenation, reducing the morphological changes and the inflammatory response. In another study 6 , the authors showed that the IPC protected against ischemia-reperfusion injury in remote lung intestinal ischemiareperfusion on or hepatic model with acute diabetes.…”

Section: Introductionmentioning

confidence: 99%

Remote ischemic preconditioning and tacrolimus in the fetal small bowel transplant in mice

et al. 2016

Self Cite

View full text Add to dashboard Cite

PURPOSE:To evaluate the effect of remote ischemic preconditioning (IPC-R) in the fetal small bowel transplantation model. METHODS:Two groups were constituted: The Isogenic transplant (ISO, C57BL/6 mice, n=24) and the allogenic transplant (ALO, BALB/c mice, n=24). In each group, the animals were distributed with and without IPC-R. It was obtained the following subgroups: Tx, IPC-R, Fk, IPC-Fk, in both strains. Intestinal grafts were stained with hematoxylin and eosin and immunohistochemically. RESULTS:The graft development evaluation in ISO group showed that IPC-R reduced the development compared with ISO-Tx and Fk: 9.0±0.6). The graft rejection was lower in groups treated with IPC-R (-18%), Fk (-68%) or both (-61%) compared with ALO-Tx. CONCLUSION:Remote ischemic preconditioning showed benefic effect even associate with Tacrolimus on the development and acute rejection of the fetal small bowel graft in the Isogenic and Allogenic transplants.

show abstract

Effects of maternal ischemic preconditioning in the colon of newborn rats submitted to hypoxia-reoxygenation insult

Cited by 3 publications

References 29 publications

Neonatal necrotizing enterocolitis rat model attenuated by a remote ischemic preconditioning in the pregnant

Neonatal necrotizing enterocolitis rat model attenuated by a remote ischemic preconditioning in the pregnant

Comparative evaluation of the osteogenic capacity of human mesenchymal stem cells from bone marrow and umbilical cord tissue

Remote ischemic preconditioning and tacrolimus in the fetal small bowel transplant in mice

Contact Info

Product

Resources

About