This study aimed to determine the effect of pH on the production of antibacterial by lactic acid bacteria. This study uses a completely randomized design factorial 3X 5 (3 X 5 treatment of bacterial isolates pH) with three replications. The first factor consisted of pH 4, pH 5, pH 6, pH 7, pH 8, and PENDAHULUANSalah satu sumber bakteri asam laktat (BAL) diperoleh dari usus itik. Pada umumnya, BAL digunakan sebagai probiotik, khususnya genus Lactobacillus dan Bifdobacterium yang merupakan bagian dari flora normal pada saluran pencernaan (Sujaya et al., 2008). Keberadaan BAL mampu bertahan di dalam variasi pH pada saluran pencernaan itik (Tillman et al., 1989). Pada tembolok itik memiliki pH sekitar pH 6,3, pH di lambung antara 1 -2, pH di ileum antara 7 dan 8, pH di proventrikulus 2 -4, dan pH di ventrikulus 2,6 (Anggorodi, 1984). Bakteri asam laktat mampu memproduksi bakteriosin untuk menghambat atau membunuh bakteri yang bersifat selektif hanya terhadap beberapa strain patogen. Salah satu produksi antibakteri dipengaruhi oleh faktor lingkungan yaitu pH, umur bakteri, waktu inkubasi, suhu dan lain -lain. PH optimum diperlukan untuk produksi antibakteri karena pH sangat berpengaruh dalam pembentukan bakteriosin optimum pada pH 5 dan 6
The aim of this study is to know the viability of Lactic Acid Bacteria isolates on ration and combination of ration with molasses. This study used a mixture of lactic acid bacteria isolates from the duck intestine (B4, B7, B8). The third bacterial isolates were inoculated on two different treatment media, on rantion media (R1), and combination of rantion + molasses (R2). The study was arranged by randomized block design (RAK) 6x2 factorial treatment pattern. Factor A is the incubation time of 0 hours, 2 hours, 4 hours, 6 hours, 8 hours and 10 hours. Factor B is two types of growing media of lactic acid bacteria, is ransum media, and combination of ransum + molasses. Each treatment was repeated 3 times. This research uses pour plate method with the calculation of the colony using colony counter. Data analyzed descriptively. The results showed that the addition of 1.6% molasses (R2) maintains the number of LAB population at 4th hour with the cell number 7,36 x 105 CFU / g. While on the ration medium (R1) can maintain the amount of LAB at 6th hour with the cell number of 6,20 x 105 CFU / g. LAB population viability on feed medium with addition of molases 1.6% (R2) has increased on storage time at 4th hour with cell number 7,36 x 105 CFU / g, while on feed medium (R1) decreased cell count 6,08 x 105 CFU / g. Keywords: Viability, Lactic Acid Bacteria, Ransum, Molase.
Penelitian ini bertujuan untuk mengetahui viabilitas Bakteri Asam Laktat dari usus itik pada dedak padi dan kombinasi dedak padi dengan molases sebagai kandidat probiotik. Penelitian dilaksanakan dalam Percobaan Rancangan Acak Kelompok yang dilakukan secara faktorial 2 x 6. Faktor A adalah 2 macam media perlakuan yaitu: media A (dedak padi) dan media B (dedak padi + molasis). Faktor B adalah lama waktu inkubasi yaitu 0 jam (kontrol), 2jam, 4jam, 6 jam, 8 jam, dan 10 jam. Masing- masing perlakuan diulang sebanyak 2 kali. Pengamatan viabilitas bakteri dilakukan dengan metode angka lempeng total untuk menentukan jumlah sel bakteri yang hidup dilanjutkan dengan menghitung viabilitas BAL. Data jumlah koloni BAL yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan isolat BAL dari usus itik pada media perlakuan dedak padi dan kombinasi dedak padi dengan molases memiliki viabilitas tertinggi pada waktu inkubasi ke- 10 jam dengan jumlah populasi sebesar Log 6,53 CFU/g pada dedak padi dan Log 6,87 CFU/g pada kombinasi dedak padi dan molases.
Penelitian ini bertujuan untuk mengetahui pengaruh substitusi tepung daun singkong terfermentasi Aspergillus niger terbaik terhadap bobot hidup, bobot karkas, dan lemak abdominal ayam joper umur 8 minggu. Penelitian ini dilaksanakan pada Januari–Maret 2022 di Laboratorium Nutrisi dan Pakan Ternak, Jurusan Peternakan, Fakultas Pertanian, Universitas Lampung dan Laboratorium Lapang Terpadu, Fakultas Pertanian, Universitas Lampung. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan (0%, 5%, 10%, 15%, dan 20%) tepung daun singkong terfermentasi Aspergillus niger yang diulang 4 kali dengan total 20 unit petak percobaan, masing-masing unit berisi 4 ekor ayam joper tanpa dibedakan jantan ataupun betina, sehingga jumlah ayam joper 80 ekor. Peubah yang diamati meliputi bobot hidup, bobot karkas, dan lemak abdominal. Data yang diperoleh dianalisis menggunakan analisis ragam (ANOVA) pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa perlakuan substitusi tepung daun singkong terfermentasi Aspergillus niger 0%, 5%, 10%, 15%, dan 20% tidak berpengaruh nyata (P>0,05) terhadap bobot hidup, bobot karkas, dan lemak abdominal ayam joper umur 8 minggu. Substitusi daun singkong terfermentasi Aspergillus niger hingga taraf 20% masih dapat ditoleransi oleh ayam joper, akan tetapi belum mampu dalam meningkatkan bobot hidup, bobot karkas, dan lemak abdominal ayam joper.
This study aimed to determine the best percentage of A. microphylla flour on carcass weight, abdominal fat percentage, gizzard weight and intestine length of broiler. This research was conducted in March--April 2021 at PT. Sinar Ternak Sejahtera farm 5 in Wonodadi Village, South Lampung Regency, Lampung Province. Carcass processing was carried out at the Livestock Production Laboratory in the Department of Animal Husbandry, Faculty of Agriculture, University of Lampung. The experimental design used was a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments were basal ration (P0), basal ration with substitution of A. microphylla 2.5% (P1), basal ration with substitution of 5% A. micophylla (P2), and basal ration with substitution of A. microphylla 7.5% (P3). The data obtained were analyzed using analysis of variance with a significance level of 5% and continued with Duncan's Multiple Range test. The results showed that the substitution of A. microphylla on carcass weight and percentage of abdominal fat had a significant effect (P<0.05) and had no significant effect (P>0.05) on gizzard weight and intestine length of broiler. Keywords: A. microphylla, Carcass weight, Abdominal fat percentage, Gizzard weight, Intestinal length
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