Ovarian cancer is the fifth most common cause of cancer deaths among American women. Platinum and taxane combination chemotherapy represents the first-line approach for ovarian cancer, but treatment success is often limited by chemoresistance. Therefore, it is necessary to find new drugs to sensitize ovarian cancer cells to chemotherapy. Persistent activation of Signal Transducer and Activator of Transcription 3 (STAT3) signaling plays an important role in oncogenesis. Using a novel approach called advanced multiple ligand simultaneous docking (AMLSD), we developed a novel nonpeptide small molecule, LLL12B, which targets the STAT3 pathway. In this study, LLL12B inhibited STAT3 phosphorylation (tyrosine 705) and the expression of its downstream targets, which are associated with cancer cell proliferation and survival. We showed that LLL12B also inhibits cell viability, migration, and proliferation in human ovarian cancer cells. LLL12B combined with either paclitaxel or with cisplatin demonstrated synergistic inhibitory effects relative to monotherapy in inhibiting cell viability and LLL12B-paclitaxel or LLL12B-cisplatin combination exhibited greater inhibitory effects than cisplatin-paclitaxel combination in ovarian cancer cells. Furthermore, LLL12B-paclitaxel or LLL12B-cisplatin combination showed more significant in inhibiting cell migration and growth than monotherapy in ovarian cancer cells. In summary, our results support the novel small molecule LLL12B as a potent STAT3 inhibitor in human ovarian cancer cells and suggest that LLL12B in combination with the current front-line chemotherapeutic drugs cisplatin and paclitaxel may represent a promising approach for ovarian cancer therapy.

BACKGROUND: To research the application of “Z” chest drainage and modified incision and closure techniques for uniportal VATS.METHODS: The 422 patients by uniportal VATS were divided into three groups:282 in experimental group(“Z” Chest drainage with two 16 F chest tube),male 156,female 126, medianage is 55 years old;100 in control group1(traditional Chest drainage with two 16 F chest tube) , male 58,female 42, medianage is 53 years old;;40 in control group2(traditional Chest drainage with two 34 F chest tube), male 24,female 16, medianage is 52 years old. The age,sex and surgical method of the three groups has no statistical significance. To compare the incidence rate of Incision exudating、Poor healing of incision and debridement between the 3 groups.RESULTS: Incidence rate of Incision exudating of experimental group, control group1 and control group2: 5（1.8%,5/282），5（5%,5.0/100） and 6（15.0%，6/40）; Poor healing of incision of the three groups: 0，1（1%,1/100） and 3（7.5%,3/40）; debridement of the three groups: 0，0 and 3（7.5%,3/40）. For the healing of incision, control group1was better than control group2;and experimental group was better than control group1.CONCLUSIONS: “Z” Chest drainage and Modified Incision and Closure Techniques decreased the incidence rate of Incision exudating、Poor healing of incision and debridement,which would be useful to obtain a better cosmetic effect after uniportal VATS .

Background Intraoperative pulmonary artery (PA) hemorrhage is one of the leading reasons for conversion from uniportal VATS to open thoracotomy ,especially for the small incision (≤ 3 cm) uniportal VATS performed by our department. So, We designed a technology called pretreatment clamping of the pulmonary artery,which may be helpful to solve the problem. Methods A retrospective analysis of 19 patients who had pulmonary artery bleeding during uniportal thoracoscopic lobectomy in which one group had undergone preventive pulmonary artery clamping, the clamping group (n = 11), and one group which did not receive preventive clamping, the non-clamping group (n = 8). We compared the rates of conversion from the uniportal VATS approach to open thoracotomy or multi-incision operation, duration of pulmonary artery repair, blood loss, length of postoperative hospital stay and postoperative complications of the two groups. Results Compared to the non-clamping group, the clamping group had lower rates of conversion to open thoracotomy (0% vs 62.5%, p < 0.05) and lower rates of conversion to multi-incision operations (18.2% of non-clamping converted to 2-port approach vs 12.5% of clamping converted to 2-port approach and 12.5% converted to 3-port approach, p < 0.05). Duration of pulmonary artery repair was reduced in the clamping group (10.1 ± 3.2 min vs 18.3 ± 5.5 min, p < 0.05). The clamping group also had decreased blood loss (23.6 ± 11.2 ml vs 47.5 ± 14.9 ml, p༜0.05). There were no significant differences in postoperative hospital stay and postoperative complications between the two groups. Conclusion Pretreatment clamping of the pulmonary artery in VATS lobectomy can decrease conversion rates, decrease blood loss, shorten repairing time of the pulmonary artery, and feasibly can be applied in uniportal thoracoscopic lobectomy.