Background Esophageal squamous cell carcinoma (ESCC) is the leading cause of cancer-related death worldwide with a poor prognosis. Given that DEPDC1B plays a key role in multiple cancers, the role of this molecule in ESCC was explored to identify potential targets for ESCC patients. Method The expression level of DEPDC1B in ESCC was revealed based on the TCGA database and immunohistochemical experiments on clinical tissues. The correlation between DEPDC1B and survival of ESCC patients was analyzed by Kaplan–Meier method. Small hairpin RNA (shRNA)-mediated silencing of DEPDC1B expression in ESCC cells and performed a series of in vitro and in vivo functional validations. Result DEPDC1B was overexpressed in ESCC. High expression of DEPDC1B was significantly negatively correlated with overall survival in patients with ESCC. Moreover, knockdown of DEPDC1B inhibited ESCC cell proliferation, clone formation, migration, tumor formation and promoted apoptosis. Furthermore, knockdown of DEPDC1B leaded to significant downregulation of GABRD in ESCC cells. Meanwhile, GABRD expression was upregulated in ESCC, and its silencing can inhibit the proliferation and migration of the tumor cells. Interestingly, there was a protein interaction between DEPDC1B and GABRD. Functionally, GABRD knockdown partially reversed the contribution of DEPDC1B to ESCC progression. In addition, GABRD regulated ESCC progression may depend on PI3K/AKT/mTOR signaling pathway. Conclusion DEPDC1B collaborated with GABRD to regulate ESCC progression, and inhibition of this signaling axis may be a potential therapeutic target for ESCC.

This study investigates the biokinetics of LGT proteome, a potential biomarker of severe TBI, in serum of severe TBI patients. The LGT proteome presents in the serum of severe TBI patients. The abundance diversity of LGT proteome is closely associated with pathologic condition of TBI patients. Serum LGT proteome may be used as a promising marker for evaluating severity of severe TBI.

BACKGROUND: To research the application of “Z” chest drainage and modified incision and closure techniques for uniportal VATS.METHODS: The 422 patients by uniportal VATS were divided into three groups:282 in experimental group(“Z” Chest drainage with two 16 F chest tube),male 156,female 126, medianage is 55 years old;100 in control group1(traditional Chest drainage with two 16 F chest tube) , male 58,female 42, medianage is 53 years old;;40 in control group2(traditional Chest drainage with two 34 F chest tube), male 24,female 16, medianage is 52 years old. The age,sex and surgical method of the three groups has no statistical significance. To compare the incidence rate of Incision exudating、Poor healing of incision and debridement between the 3 groups.RESULTS: Incidence rate of Incision exudating of experimental group, control group1 and control group2: 5（1.8%,5/282），5（5%,5.0/100） and 6（15.0%，6/40）; Poor healing of incision of the three groups: 0，1（1%,1/100） and 3（7.5%,3/40）; debridement of the three groups: 0，0 and 3（7.5%,3/40）. For the healing of incision, control group1was better than control group2;and experimental group was better than control group1.CONCLUSIONS: “Z” Chest drainage and Modified Incision and Closure Techniques decreased the incidence rate of Incision exudating、Poor healing of incision and debridement,which would be useful to obtain a better cosmetic effect after uniportal VATS .