Arabica coffee (Coffea arabica L.) ground nanoparticles contain phenolics compounds that have anti-inflammatory effects, so they can be used as sources of active compounds in anti-inflammatory gel preparations. This study aims to determine the optimum formulation of anti-inflammatory gel preparations using Arabica coffee ground nanoparticles as active compounds. Treatment optimization was performed using a Response Surface Methodology according to the Box-Behnken Design with a quadratic model in the Design Expert Version 10.0.3.0 software. In this study we used three factors (x): carbopol 940, triethanolamine (TEA), and nanoparticles, each of which consists of three levels, the response (y) observed including the acidity degree (pH), spreadability, viscosity and total phenolic content. ANOVA analysis results show that the quadratic model is very appropriate since it produces a high R2 value and a low PRESS value for all responses, as well as significant p-values (<0.0500) and an insignificant lack of Fit values (p-value> 5%). The optimum formulations for the gel preparations of the Arabica coffee ground nanoparticles obtained in this study are carbopol 940 (0.569%), TEA (0.468%), and nanoparticles (3.000%), which have values w/o an interval (0.994) and a desirable (0.981) response to acidity (5.212), spreadability (5.850 cm), viscosity (3734.244 cps) and total phenolic content (669.227 µgGAE/g).

Arabica coffee (Coffea arabica L.) grounds are kind of solid waste produced from filtering process containing many bioactive components and good for health. Nanoparticle technology can be used to produce nanoscale particles without separating the active compounds contained the rein. The aim of this study is to determine the potential of Arabica coffee grounds nanoparticles as active compounds to be used in pharmaceutical preparations. The research procedure was begun with collect the Arabica coffee grounds which was then processed into nanoparticles using the ball milling and ultrasonication technique. The nanoparticles were characterized using some techniques, namely particle size analysis using PSA (Particle Size Analyzer), phytochemical screening, total phenolics, pH, particle morphology using SEM (Scanning Electron Microscopy) and particle structure using FT-IR (Fourier Transform Infrared Spectroscopy). The results showed that Arabica coffee grounds nanoparticles has a particle size of 396.0 nm with a polydispersity index of 0.254, solubility of 70.680% and pH 5.33, the positive Arabica coffee grounds nanoparticles containing secondary metabolites of alkaloids, saponins and phenolics with total phenolics 1246.90 μgGAE/g. Morphology of Arabica coffee grounds nanoparticles was in irregular granules form with an asymmetrical size and has active functional groups such as OH, NH, CH alkane, C≡N, C=O carboxylic acid, -C=C-, CO, and CC. It can be concluded that Arabica coffee grounds nanoparticles were potentially can be used as active compounds in various pharmaceutical preparations.

Penelitian ini bertujuan untuk mengetahui kemampuan proses Fenton dalam menurunkan kadar chemical oxygen demand (COD) dan kadar total suspended solid (TSS) dari limbah cair pabrik kelapa sawit (PKS) dan menentukan kondisi optimum dari parameter yang digunakan dengan Response Surface Methodology menurut Box- Behnken design. Sampel diambil pada keluaran pertama kolam anaerobik ketiga dari instalasi pengolahan limbah cair kelapa sawit yang mengandung nilai COD berkisar antara 8.000 hingga 12.000 ppm. Pada penelitian ini, dilakukan pengujian pada berbagai pH, konsentrasi FeSO4.7H2O dan konsentrasi hidrogen peroksida. Hasil  penelitian menunjukkan bahwa kemampuan proses AOP dengan metode Fenton dapat menurunkan konsentrasi COD dan TSS masing-masing adalah 70,7704% dan 88,3897% pada konsentrasi FeSO4.7H2O 3703,52 ppm, konsentrasi H2O2 5586,43 ppm, dan pH 3.

Background: Arabica coffee ground nanoparticles gel is known to possess good physicochemical properties and high phenolic contents in which the phenolics are famous for their anti-inflammatory activities. Objectives: : This study aimed to investigate the anti-inflammatory activities of the arabica coffee ground nanoparticles gel on carrageenan-induced male mice for 6 h. The positive control was commercial medicine (Voltaren gel), and the control negative was base gel. Methods: The inflammatory inhibition of arabica coffee ground nanoparticles gel is determined by measuring the edema volume of the carrageenan-induced mice foot using a plethysmometer. Anti-inflammatory activities of arabica coffee ground nanoparticles gel were optimized using Box-Behnken Design of the response surface methodology. Results: The anti-inflammatory activities or inflammatory inhibition of arabica coffee ground nanoparticles gel was determined by measuring the edema volume of the carrageenan-induced mice foot. The increase of edema volume of the mice foot from the first to 180th min was caused by the release of inflammation mediator such as histamine, prostaglandins, bradykinins, and serotonin on the tissue; meanwhile, at the 240th min, the edema of the mice foot experienced a decrease due to the inhibition of prostaglandins synthesis to the tissue. The obtained results indicated that the arabica coffee ground nanoparticles gel has average value of anti-inflammatory activities of 27.75%. The obtained optimum formulation is 0.500% Carbopol 940, 0.400% triethanolamine, and 2.313% nanoparticle, giving 29.360% and 29.670% for the predicted and experimental inflammatory inhibition values, with 0.812 desirability value, respectively. Conclusions: The Box-Behnken design can be used to determine the optimal gel formulation of arabica coffee grounds for inflammatory inhibition.

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