absorption of calcium and phosphate. Vitamin D was long known to be a major player in calcium homeostasis and bone metabolism. Vitamin D was first discovered at the beginning of the 20th century as a missing nutrient in children with severe bone demineralization, a disease called rickets. In humans, the most important related compounds of vitamin D are vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). [1] Vitamin D2 is synthesized by plants (mainly mushrooms and yeast), whereas vitamin D3 is synthesized in skin when it is exposed to ultraviolet B rays from sunlight or from artificial food source such as fatty fish, eggs, beef liver, and mushrooms. So, it is also called " sunshine vitamin." [2] Background: Vitamin D is a group of fat-soluble secosteroids responsible for intestinal absorption of calcium and phosphate. Vitamin D is a major player in calcium homeostasis and bone metabolism. In humans, the most important compounds of vitamin D are vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Most of the vitamin D3 is synthesized in skin when it is exposed to ultraviolet B rays from sunlight. Vitamin D deficiency causes osteomalacia in adults and rickets in children. Deficiency of vitamin D results in impaired bone mineralization and bone damage, which leads to bone-softening diseases. Objective: To find out the prevalence of vitamin D deficiency in well socioeconomic population of Ahmedabad,