The efficacies of many indigenous plants for several disorders have been practioners of traditional herbal medicines (Muhammad and Muhammad, 2005). These plants provide on interesting and still largely unexplored source in the creation and the development of potential new drugs for chemotherapy which might help to overcome the growing problem of resistance and the toxicity of the currently availa antibiotics (Rahmoun et al., 2013). Lawsonia inermis Linn. (Lythracea a biennial dicotyledonous herbaceous shrub. Being native of North Africa and South Asia, the plant is now widely cultivated throughout the tropics as an ornamental dye plant (Gagandeep et al., 2010). Henna has been used cosmetically and medi other 9,000 years. Henna leaves, flowers, seeds, stem bark and roots are used in traditional medicine to treat a wide variety of ailments which include rheumatoid arthritis, headache, ulcers, diarrhea, leprosy etc. The leaves are used in alleviating skin diseases (Gagandeep et al., 2010). medicinal method, especially the use of medicinal plants, still plays a vital role to cover the basic health needs in the developing countries. Therefore, it is of great interest to ABSTRACT Lawsonia inermis (henna) has been widely used over centuries for medication and cosmetics in some regions of the world. The leaves and seeds of henna are known for alleviating a number of skin diseases including extracted using maceration method. screening of aqueous, ethanolic and petroleum ether extracts of leaves of Lawsonia inermis against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, albicans and Epidermophyton floccosum were investigated using the agar well diffusion method. The phytochemical screening showed the presence of saponins, flavonoids and steroids in all the extracts. All tested isolates were susceptible to all the 1000µg/ml. The highest activities were observed in the aqueous extract of the plant against Staphylococcus aureus and Epidermophyton floccosum (19mm). While the standard drugs active against the same isolates at 20 µg/ml with zones of 28mm andThe findings of this study suggest that Lawsonia inermis could serve as a potential antimicrobial agent and provide the basis for isolation and identification of biologically active constituents in these extracts.

According to World Health Organization reports, there is a major health challenge now and in the future due to increase resistance of bacteria to well-known antibiotics. This study aimed at identifying some vital metabolites from Hexane fraction of clove ( Syzygium aromaticum) using Liquid Chromatography Mass Spectrometer (LCMS) and its antibacterial activity against some foodborne pathogens. Syzygium aromaticum buds were extracted with Hexane using maceration method. This was followed by LCMS analysis for s econdary metabolites and then antibacterial activity using Agar well diffusion against four foodborne pathogens (Enterobacter spp, Salmonella spp , Escherichia coli, and Staphylococcus aureus). The Minimum inhibitory concentrations (MICs) and Minimum Bactericidal (MBC) of the clove extracts were also determined by the micro dilution method. The LCMS profile revealed the presence of significant metabolites such as Nevadensin (8 O methylated flavonoids) and 9 Hydroxymegastigma 4, 6,7 trien 3 one (Sesquiterpe noids) among others belonging to the well-known class of bioactive compounds that shows antioxidant and anti-inflammatory potentials. Based on the results obtained, Enterobacter spp was found to be the most susceptible organism with an average zone of 12.5 0±1 .20 mm, followed by E. coli 10.50±1.20 mm. The MIC and MBC of the extract against the test organisms ranged from 62.5 to 2 50 µg/ml. Therefore, the antibacterial activities observed might be as a result of these metabolites, and could be recommended for used in drug management or as antibacterial agent.

Mint herbs have been reported to possess several biological effects, and the dried leaves are traditionally used for herbal tea or medicine. Extraction of plant materials was by maceration method. Both preliminary qualitative screening of phytochemicals and Liquid Chromatography Mass Spectrometer (LCMS) profiling revealed many useful metabolites. The phytochemicals include: Saponin, Carbohydrate, Alkaloids, Cardiac glycoside, and Steroid. Some LCMS compounds among others were also evaluated such as Quassin, Epioxylubimin, furocoumarinic acid glycoside and Dioctylamine. The antimicrobial activity of Mentha piperita extract against some food-borne pathogens such as Escherichia coli, Salmonella spp., Enterobacter spp. and Staphylococcus aureus were evaluated using agar well diffusion method at different concentrations ranging from 2000 µg/ml - 250µg/ml. The results revealed that hexane mint extracts had activity against the bacterial isolates. The highest activity was against E. coli (11.50 ±1.50) while the least activity was against Enterobacter spp (6.14 ± 0.13). The potential bioactive metabolites identified could be responsible for the antimicrobial activities observed. These findings have thus proven that Mentha piperita is more effective against E. coli. Therefore this extract could be used in the control and prevention of many food-borne pathogens and can be of importance in developing new biopreservatives. Key words: Metabolite, phytochemical, Mint, Antimicrobial, Pathogen.