The efficacies of many indigenous plants for several disorders have been practioners of traditional herbal medicines (Muhammad and Muhammad, 2005). These plants provide on interesting and still largely unexplored source in the creation and the development of potential new drugs for chemotherapy which might help to overcome the growing problem of resistance and the toxicity of the currently availa antibiotics (Rahmoun et al., 2013). Lawsonia inermis Linn. (Lythracea a biennial dicotyledonous herbaceous shrub. Being native of North Africa and South Asia, the plant is now widely cultivated throughout the tropics as an ornamental dye plant (Gagandeep et al., 2010). Henna has been used cosmetically and medi other 9,000 years. Henna leaves, flowers, seeds, stem bark and roots are used in traditional medicine to treat a wide variety of ailments which include rheumatoid arthritis, headache, ulcers, diarrhea, leprosy etc. The leaves are used in alleviating skin diseases (Gagandeep et al., 2010). medicinal method, especially the use of medicinal plants, still plays a vital role to cover the basic health needs in the developing countries. Therefore, it is of great interest to ABSTRACT Lawsonia inermis (henna) has been widely used over centuries for medication and cosmetics in some regions of the world. The leaves and seeds of henna are known for alleviating a number of skin diseases including extracted using maceration method. screening of aqueous, ethanolic and petroleum ether extracts of leaves of Lawsonia inermis against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, albicans and Epidermophyton floccosum were investigated using the agar well diffusion method. The phytochemical screening showed the presence of saponins, flavonoids and steroids in all the extracts. All tested isolates were susceptible to all the 1000µg/ml. The highest activities were observed in the aqueous extract of the plant against Staphylococcus aureus and Epidermophyton floccosum (19mm). While the standard drugs active against the same isolates at 20 µg/ml with zones of 28mm andThe findings of this study suggest that Lawsonia inermis could serve as a potential antimicrobial agent and provide the basis for isolation and identification of biologically active constituents in these extracts.