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Moringa (Moringa oleifera) has frequently been studied as an alternative and promising protein source in fish feed. Moringa leaves is among the potential and affordable plant-based protein source ingredient, as it protein, vitamin, beta-carotene, and various kind of minerals. Flavonoid, saponin, vitamin and alkaloids reportedly exhibit immune-stimulating activity which increase immune response. This study aims to evaluate the replacement of fish meal with fermented Moringa leaves meal in feed on the immune response of red tilapia (Oreochromis sp.). The research was conducted experimentally using a completely randomized design by four treatments in triplicate. The treatments include replacement of the fish meal with fermented moringa leaves meal at 0, 10, 20, and 30 %. Red tilapia with total length of 9 to 10 cm was fed twice daily for two months at a feeding rate of 3 % of total biomass. The results showed that replacement fish meal with fermented Moringa leaves meal as much as 20 % increases the percentage values of hematocrit and leukocrit, stimulates phagocytic activity and phagocytic index, suppresses monocyte and increases lymphocytes, and increases total plasma protein.

This study was carried out to evaluate the effects of probiotic dosage on water quality, total number of Aeromonas spp. and Pseudomonas spp., in eel (Anguilla sp.) cultivation. Experiment was conducted by using fiber glass tanks (1x1 m 2 ) with 800 L water volume. Eel fingerling with 50 g average body weight were stocked at a density of 20 eel/tank in triplicates. Observation was conducted on total number of bacteria and water quality. The results indicated that the total bacterial count increased gradually by the time of cultivation with highest density of 1.49x10 7 cfu/mL. Probiotics at a dose of 40 mL/tank weekly increased the total count of bacterial cells in water. Probiotic application was able to decrease the total number of both Aeromonas spp. from 1.95 x 10 5 to 5.44 x 10 4 cfu/mL, and Pseudomonas spp. from 3.57 x 10 4 to 8.1 x 10 3 cfu/mL. Water quality during this study was not significantly affected by the application of probiotic. This result suggested that the application of proper dose probiotics suppresses Aeromonas spp. and Pseudomonas spp. cells density in water, and might decrease the bacterial infections in eel culture.