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The effects of ionic liquid media on the activity of Candida antarctica lipase B in a simple transesterification reaction were studied. In ionic liquids containing alkylsulfate, nitrate and lactate anions, which dissolved CaLB, the reaction was at least ten times slower than in [BMIm][BF 4 ]. Only [Et 3 MeN][MeSO 4 ] was an exception, as dissolved CaLB maintained its activity in this solvent. By means of FT-IR spectroscopy, denaturation of CaLB was observed upon dissolution in ionic liquids in which the activity was low, whereas the conformation of enzyme dissolved in [Et 3 MeN][MeSO 4 ] closely resembled the native one.

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biochemical syntheses, microbiological syntheses biochemical syntheses, microbiological syntheses O 0035 -039Lipase-Catalyzed Reactions in Ionic Liquids.-Preliminary studies concerning transesterification of ethyl butanoate, transesterification and ammonolysis of ethyl octanoate and epoxidation of cyclohexene are presented.The reactions are carried out with Candida antarctica lipase in the presence of 1-butyl-3-ethylimidazolium tetrafluoroborate or hexafluorophosphate. The rates are comparable with or better than those obtained in organic media. -(LAU, R. MADEIRA; VAN RANTWIJK, F.; SEDDON, K. R.; SHELDON, R. A.; Org. Lett. 2 (2000) 26, 4189-4191; Lab. Org. Chem. Catal., Delft Univ. Technol., NL-2628 BL Delft, Neth.; EN)