No abstract

Low-level laser therapy (LLLT) is a form of phototherapy used to promote cell proliferation. This study investigates the potential role of LLLT in cellular proliferation of human monocytic leukaemia cells (THP-1) under in vitro conditions. Cells were irradiated with an 850 nm diode laser and exposed to doses ranging from 0-26.8 J/cm 2. After irradiation, cells were incubated for 12 hr and 24 hr to allow time for proliferation. Comet assay was conducted to evaluate genotoxicity of the irradiated cells. Trypan blue was used to estimate cytotoxicity, which peaked at the highest dose as expected. Preliminary results suggest that cell counts increase at low doses, whereas a decrease in cell number at high doses was noted compared to controls. Comet assay showed no significant difference between irradiated and non-irradiated cells at low doses. In contrast, DNA damage increased at doses ≥ 8.9 J/cm 2 and was comparable to the 100 µM H 2 O 2 positive control at the highest fluence. It could be concluded that LLLT has the ability to stimulate the THP-1 cell line to proliferate if supplied with the correct energy and dose.

Photobiomodulation (PBM) is a form of the use of visible red and Near-infrared (NIR) light at low power, where a laser light photon is absorbed at the electronic level, without heat production. PBM can be applied in wide range of treatment to help the wound, inflammation, edema, and pain reduction. However, there is a lack of scientific documentation regarding its actual effects. Objectives: This study assesses the impact of PBM on the release of M1-related cytokine in monocyte cells with particular emphasis on interleukin-1β (IL-1β) and Tumour Necrosis Factor α (TNF-α). Methods: Tamm-Horsfall Protein 1 (THP-1) macrophages M1 cells have been exposed to the light from the diode laser of 850nmat different doses (0, 0.6, 1.2 and 3.6 J/cm2). The release of cytokines was determined by enzyme-linked immunosorbent assay, after different periods of incubation (0, 12, 24, and 48 hours) post-irradiation. The proliferation of fibroblast cells suspended in irradiated M1-supernatent was evaluated for the same periods of incubation. Results: The results showed that PBM significantly enhanced M1-related cytokine release (p < 0.05). Obviously, IL-1β increased post-irradiation at 1.2 J/cm2 more than other doses for all incubation periods. TNF-α was decreased significantly after two days of irradiation (p < 0.005) for all doses. A significant increase in fibroblast proliferation (p < 0.005) was observed concomitant with the boost of cytokine release. Conclusion: This in vitro study has demonstrated that the PBM of the 850 nm diode laser therapy can enhance M1-related cytokine release, which in turn increases the proliferation of fibroblast cells. Moreover, PBM at 850 nm plays an anti-inflammatory role, which manifested by decreasing the level of TNF-α. Therefore, this therapy may be able to accelerate the wound healing process.

Background: Accurate measurement of a patient’s height and weight is an essential part of diagnosis and therapy, but there is some controversy as to how to calculate the height and weight of patients with disabilities. Objective: This study aims to use anthropometric measurements (arm span, length of leg, chest circumference, and waist circumference) to find a model (alternatives) that can allow the calculation of the height and the body weight of patients with disabilities. Additionally, a model for the prediction of weight and height measurements of patients with disabilities was established. Method: Four hander patients aged 20-80 years were enrolled in this study and divided into two groups, 210 (52.5%) male and 190 (47.5%) female. Result: A significant correlation was noted between body height and arm span, as well as between body height and length of leg in all study groups. The body weight and the ratio of arm span or leg length to the sum of chest and waist circumferences were found to have a negative significant correlation. Model equations were derived to estimate the height and body weight according to anthropometric measurements. Conclusion: Anthropometric measurements can be used to create a model for calculating the body height and body weight of patients with disabilities and which can be considered an alternative to measurements that can be made on otherwise healthy subjects.