The present paper features processes of serum biotransformation. The serum was obtained from triticale extract and pea flour after protein concentrates of increased biological value had been extracted. The research objective was to obtain microbial and vegetable feed concentrates by using a composition of Saccharomyces cerevisiae121 yeast and the yeast-like fungus Geotrichumcandidum 977. The mass fraction of protein in the two-component composites was 75–80% of the dry matter. The score of the first and the second limiting amino acids (lysine and threonine) equaled 103–113%, and that of the third acid (sulfur- containing) was 71–72%. The chemical composition of the composites corresponded to the ‘Concentrates’ group; the values of their functional and technological properties were typical of concentrates from other types of grain crops. The study revealed some cultures that are able to actively develop in serum, which is a secondary product of processing the extract after protein isolation. A symbiotic ferment was prepared from the fungus Geotrichumcandidum 977 and the yeast Saccharomyces cerevisiae 121, which ensures the growth of biomass in a carbohydrate- and nitrogen-containing medium. Proteins were isolated under the action of amylase, glucoamylase, cellulose, and xylanase. The amount of high-molecular compounds (dextrins) and trioses (raffinose) released from the interaction with protein and non-starch polysaccharides decreased 2–4 times in the solution. The amount of glucose, disaccharides, xylose, and galactose increased 2–10 times, compared with the original extracts. The serum remaining after the removal of the main mass of the protein was enriched with low molecular weight mono- and oligosaccharides, which positively affected the growth of microorganisms. The mass fraction of proteins in the microbial-vegetable composite obtained from the extract with the triticale proteins and pea flour ratio of 1:5 was 15% higher than at the ratio of 1:3. Microbial and vegetable concentrates with a mass fraction of protein of 55.8–75.1% of dry matter can be used in fodder production as a protein-carbohydrate additive. Protein composites made of protein triticale and peas with a complementary amino acid composition can improve the biological value and performance of food products.
The process of bioconversion of the secondary product of pea flour processing into protein concentrate (serum) into fodder microbial-plant concentrate has been optimized. For this, a composition was selected from the culture of the fungus Geotrichium candidum 977 and the yeast Saccharamyces cerevisiae 121, a mathematical model of concentrate synthesis was developed in the form of an equation that adequately describes the dependence of the biomass yield on technological parameters: pH of the medium, temperature and amount of inoculum. The concentrate from the biomass had a protein mass fraction of 61.68 % of DS, from the biomass with the culture liquid - 57.90 %. Concentrates - biologically complete, the rate of essential amino acids was 107-226 %, out of 30 fatty acids, 97 % were acids that are part of animal fats, vegetable oils or marine organisms. The ratio of saturated and unsaturated acids is 1:3, the content of trans isomers is 5.1 %, omega-6 fatty acids (linoleic) is 19.73 %. The ability of symbiosis between the yeast S. cerevisiae 84/5 and the fungus Trich. cutaneum 656 has been proven. transform the components of whey remaining after the extraction of phytin from rice bran into protein biomass. The ratio of monocultures by mass fraction is 1:1, pH - 5.0…6.0, duration of growth - 72 hours, digestibility – 90 %. The protein is enriched with methionine, isoleucine, leucine, lysine. The amount of essential acids is 18-21 % higher than in concentrates obtained from individual monocultures. The use of concentrates is advisable to use in the diet of animals.
Studies on the bioconversion of whey water formed from chickpea and pea grains in the preparation of protein concentrates have been performed. The serum remaining after precipitation of the main part of the protein was subjected to a symbiotic transformation of Saccharomyces cerevisiae 121 and Geotrichum candidum 977 yeast cultures with the formation of protein-containing products with a mass fraction of protein (52.27-57.90% of DS) and a complementary amino acid composition. A microbial-plant concentrate was used as an additive in the feeding of Wistar laboratory rats. After 25 days of feeding, there was no negative effect on the physiological parameters and behavior of animals, which indicates the high quality of the protein product and the prospects of its inclusion in the composition of animal feed and diets.
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