This research was carried out at the Plant Tissue Culture Laboratory (PTCL) in Faculty of Environmental Agricultural Sciences, Arish University, Egypt during 2013 to 2016. The aim of this study was using tissue culture technique for the micropropagation of Origanum syriacum L. plant. One node cutting and shoot tip were cultured on Murashige and Skoog (MS), Nitsch and Nitsch (NN) and Gamborg (B5) media. The results showed that shoot tip cultured on the MS medium had the best combination for the establishment stage of mother plants. Shoots cultured on MS medium supplemented with0.50 mgl -1 Kin and 0.05 mgl -1 NAA produced the highest shoots developed during multiplication stage. MS medium supplemented with 0.40 mgl -1 thiamine achieved the best shoots development compared with the other additive treatments and control. Addition of 1 mgl -1 IBA to the full strength MS medium and 1.00 mgl -1 IAA to the half strength MS had the highest number of main roots and root lengths. Hardening the produced rooted plantlets were done in a greenhouse in pots containing mixture of peatmoss, vermiculite and washed sand at the rate (1:1:1) and after adjustment of mixture pH. Plantlets were successfully acclimated with 93 % survive.

Effect of three medium types, Murashige and Skoog (MS), Linsmaier and Skoog (LS) and Lloyd and McCown (WPM), two explant types (shoot-tip and single node cutting), three cytokinin types (Benzyladenine, Kinetin and 2-isopenteny1 adenine), two additives; viz., s (thiamine and pyridoxine hydrochloride) and amino acids (asparagine and arginine) and two gelling agents (guar seed powder and locust bean seed powder) combined with agar on micropropagation of Haplophyllum tuberculatum were investigated. For rooting, the effect of three auxins (Indole acetic acid, Indol butyric acid and Naphthalene acetic acid) were studied in combination with three medium strengths (full, half and quarter) combined with the best mixture (0.50 mgl-1 Kin + 6 gl-1 agar with 2 gl-1 guar seed powder) of gelling agents. Obtained results showed that Murashige and Skoog (MS) was the best medium with single node explant as starting material during establishment stage. Multiple shoots were obtained on MS medium supplemented with 1.0 mgl-1 Kinetin (Kin) combined with 0.1 mgl-1 α-naphthalene acetic acid (NAA). Thiamine and arginine were the best vitamins and amino acid at 0.1 mgl-1 and 0.50 mgl-1 , respectively. Agar at 6 gl-1 + guar seed powder at 2 gl-1 was the best gelling agent treatment. Half strength MS medium with 0.50 mgl-1 Kin + 0.5 mgl-1 NAA and 6 gml-1 agar with 2 gml-1 guar seed powder was the best combination for rooting were successfully (85% survival) acclimatized in mixture of peatmoss, vermiculite, washed sterilized sand and perlite (1:1:1:1, V/V/V/V).

The present work aimed to improve lavender plants (Lavandula officinalis L.) and volatile oil by using N fertilization and dry yeast in field experiment during the two seasons of 2013/2014 and 2014/2015. Four rates of nitrogen fertilizer were used in this work; 0, 50, 100 and 150 kg/fed as urea (46 %N) combined with four dry yeast concentrations (0%, 10%, 20% and 30%). The results were recorded that vegetative growth (plant height, herb fresh and dry weights/plant), root system (fresh and dry weights of root/plant), oil yield (oil yield/plant and per fen) and some chemical constituents (N, P and K percentages). Generally the highest values of vegetative growth and oil content per plant and per fed were obtained by using urea at level (150 kg.fed -1 ) combined with spraying plants' leaves by 30% dry yeast as foliar spry under North Sinai conditions.

during two successive seasons 2017-2018 and 2018-2019 to study the effect of various levels of nitrogen fertilizer (0, 100, 200 and 300 kg fed -1 ) in combination with different rates of humic acid (0, 150, 250 and 350 ppm) as a foliar spray on plant growth, flowering characteristics, corms and cormels characteristics and chemical constituents. The obtained results cleared that using 200 kg fed -1 nitrogen in urea forms with 350 mgl -1 humic acid as a foliar spray gave the maximum value in each of vegetative growth parameters, flowering characteristics, diameter of corms, weight of cormel, and chemical constituents (N, P and K) content in leaves. However, a non-significant variation was observed in the treatment 200 kg fed -1 nitrogen with the three concentrations of humic acid for weight of corms and No. of cormels in plants in most cases. While, plants grown without nitrogen and humic acid (control) gave the minimum values in all parameters.Humic acid is a natural organic polimar composition produced as a result of decaying organic matter, peat as well as lignin, and so that can be used to increase productivity and its quality (Valdrighi et al., 1996). The positive effects are directly on the plant growth and improve the growth of shoots and roots, absorpotion of nitrogen, calcium, potassium, magnesium and phosphorus by plant (Haghighi et al., 2011). The application of humic acid

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