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The article studies the impact of one-time physical activity of varying intensity on the secretory activity of neutrophils and monocytes in the blood of athletes of different sports specialization. Found that a single physical load average and peak levels experienced by athletes involved in aerobics, football and ran middle distance, cause a similar decrease in the expression of the secretory activity of neutrophils and monocytes in the blood, which is a decrease in the secretion of IL-1β, IL-6, IL-8, TNF-α and αIFN. Single physical loads threshold adverse effect on the functional activity of neutrophils and monocytes in athletes of different specialization do not have.