This research was carried out with the aim of phytochemical analysis and determining antioxidant activity present in methanol and chloroform leaf extracts of Azadirachta indica. Due to its potential in curing various ailments as well as wide spread application of antioxidant activity such as in the field of cosmetology, the plant was selected for the study. The total phenolics contained in the plant extracts were also studied which are responsible for the antioxidant activity. Antioxidant activity of the extracts were evaluated by diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method using ascorbic acid as standard in the concentration of 100, 50, 25 and 12.5 µg/ml. Phytochemical analysis were done with the established procedure and total phenolic content (TPC) was determined by using FolinCiocalteu colorimetric method. Phytochemical screening revealed the presence of similar constituents in both methanol and chloroform extracts such as alkaloids, glycosides, carbohydrate, phenol, flavonoid, steroids, protein, and amino acids. Total phenolic content in methanol and chloroform extracts were 207.39 ± 8.77 and 58.08 ± 4.41 mg gallic acid equivalent (GAE)/g, respectively. The inhibitory concentration (IC 50 ) value for methanol and chloroform extracts of A. indica were calculated and found to be 80.28 and 439.60 µg/ml, respectively. The finding suggests that methanol extract of the plant has significantly more antioxidant activity than the chloroform extract as clarified by total phenolics contained in the plant.

The present study was carried out to evaluate and compare anti- carrageenan induced rat paw edema was adopted for the study of anti-inflammatory activity where adult Wistar albino rats (110-160)gm of either sex were selected for study. Adult rats were divided into 10 groups, carrageenan was injected in hind paw and treated with extract at the standard dose of 200 mg/kg and 400mg/kg. The paw volume was measured at different time intervals i.e. 0,2,4 and 12 hours. Percentage inhibition of inflammation was proportional with the total phenolic content. The result indicates that methanol extract of A. indica has highest anti-inflammatory activity than both extract of J. adhatoda while its chloroform extract has least activity among all extracts.