Objective: A new simple, precise, rapid and selective high -performance thin-layer chromatographic (HPTLC) method has been developed for th e determination of lupeol in methanolic leaves extract of Andrographis echioides leavesResults: The retention factor of lupeol was found to be 0.55. Linearity was obtained in the range of 5000 ng -10000 ng for lupeol. The developed and validated HPTLC method was employed for lupeol in methanolic leaves extract of Andrographis echioides for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99917 for lupeol. Satisfactory recoveries of 99.80 % were obtained for lupeol.. The concentration of standard lupeol was 1 mg/ml. Conclusion:The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of lupeol in methanolic leaves extract of Andrographis echioides.

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Plants are the crucial resources and backbone of human life on earth and another main significance of plants is their medicinal properties. Medicinal plants provide an estimable source of lead compounds for the discovery of noble new drugs with few undesirable side effects. In this study, the Qualitative phytochemical analysis, quantitative estimation, and proximate analysis of phytochemical compounds with the importance pharmacological significance in various organic solvent extracts of Andrographis echioides leaves. Preliminary phytochemical screening analyses were determined using standard protocol methods. Qualitative phytochemical analysis of leaves extracts from Andrographis echioides was analyzed for the primary and secondary metabolites of phytochemical constituents such as sugar, protein, amino acids, Carbohydrates, alkaloid, Flavonoids, Terpenoids, Glycosides, Tannins, Gum, Mucilage, phenolic compounds, Phytosterols, Coumarin, Emodin, Saponin, Oils, and Fats were screened in different solvent extracts. Quantitative estimation of secondary metabolites from Andrographis echioides leaves are determined by using Standard methods and proximate analysis of fibre, protein, fat, total ash, and carbohydrate were carried out by using standard. Among the nine different extracts of Andrographis echioides ,methanolic extract of the leaves showed the maximum amount of phytochemical was screened in Andrographis echioides. Quantitative estimation of leaves from Andrographis echioides contained maximum content of total phenols, flavonoids, Steroids, and Terpenoids with the minimum amount of saponins, alkaloids, and Tannin. The proximate analysis in percentage showed that Andrographis echioides leaves had the highest amount of Carbohydrates content 33.78% and Protein content 27.21%. and the lowest amount of Fibre content 10.56% Fat content 1.13% and ash content 7.32%. The presence of phytochemical compounds supports the traditional use of Andrographis echioides as an alternative treatment for curing certain health conditions. Further studies should be carried out to isolate specific phytochemical chemical constituents and should be used in different studies to explore their Biological effects.

Objective: The purpose of this study is to isolate and characterize the andrographolide and betulin from methanolic leaves extract of Andrographis echioides and also used to evaluate the alpha-amylase and alpha-glucosidase inhibitory activity of isolated compounds using in silico docking studies. Methods: The isolation was done using column chromatography using gradient mobile phase. Structural elucidation was carried out on the basis of spectral analysis. In this view, andrographolide and betulin were prepared for the docking evaluation. In silico docking studies were carried out using a recent version of Auto Dock 4.2, which has the basic principle of Lamarckian genetic algorithm. Results: On the basis of the spectral data, the compounds have been established as andrographolide and betulin are being reported from this plant for the first time. The result showed that the andrographolide showed a binding affinity for amylase: (-7.9 kcal/mol) and for glucosidase (-7.2 kcal/mol) while betulin showed (-8.6 kcal/mol) and (-5.2 kcal/mol), respectively. Conclusion: Therefore, it is suggested that isolated compounds andrographolide and betulin contributed excellent α-amylase and α-glucosidase inhibitory activity because of its structural parameters. Thus, these isolated compounds can be effectively used as drugs for treating diabetes which is predicted on the basis of docking scores.