HPTLC Method for the Determination of Lupeol From Andrographis Echioides Leaves

Gurupriya, S; Cathrine, L.; Pratheema, P.

doi:10.22159/ijpps.2018v10i5.24748

Cited by 8 publications

(7 citation statements)

References 15 publications

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“…The reductors lend electron to the radicals and thereby cease the radical chain reaction forming stable compounds. The study revealed that the extract possesses similar reducing power compared with ascorbic acid as standard [25].…”

Section: Discussionmentioning

confidence: 89%

High-Performance Thin-Layer Chromatography Analysis and Free Radical Scavenging Potential of South Indian Orthodox Black Tea

Rajamani¹,

Krishnasamy²,

Nishadh

2018

Asian J Pharm Clin Res

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Objective: The objective of this study was to evaluate the free radical scavenging potential and high-performance thin-layer chromatography (HPTLC) fingerprinting of the ethanolic extract of south Indian orthodox black tea (OBT).Methods: Phytochemical analysis was carried out using standard methods, and free radical scavenging activity of the extract was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), superoxide anion (SOD), and hydroxyl radical scavenging capacities. The ethanolic extract of OBT was loaded in the pre-coated HPTLC plates (silica gel 60 F 254) E-MERCK KGaA. HPTLC was carried out with toluene: ethyl acetate: diethylamine (7:2:1), chloroform: methanol:formic acid (8.5:1.0:0.5), and butanol: isopropyl alcohol (1:1) as mobile phase for alkaloids, flavonoids, and terpenoids, respectively.Results: HPTLC results confirmed that the extract contained several potential active components such as flavonoids, alkaloids, and terpenoids as the slides revealed multicolored bands of varying intensities. Extract of OBT reliably showed the total phenolics 132.27 mg/g, flavonoids 72.52 mg/g, and alkaloids 66.01 mg/g of dry matter. The IC50 value of OBT for DPPH was found to be 372.22 μg/ml, SOD 311.93 μg/ml, NO 362.17 μg/ml, hydroxyl radical 342.14 μg/ml, and reducing power 178.54 μg/ml.Conclusion: The HPTLC fingerprinting profile developed for ethanolic extract will help in proper identification and quantification of marker compounds. The ethanolic extract of OBT was found to possess a wide range of phytochemicals with excellent antioxidant properties. This information may help to choose the best beverage to be consumed in the future.

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Section: Discussionmentioning

confidence: 89%

High-Performance Thin-Layer Chromatography Analysis and Free Radical Scavenging Potential of South Indian Orthodox Black Tea

Rajamani¹,

Krishnasamy²,

Nishadh

2018

Asian J Pharm Clin Res

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“…Previously some methods were reported on Bauhinia plant species but they have reported some other compounds such as syringic acid, vanillic acid, caffeic acid, and kaempferol (Gupta et al, 2015. Some studies have been reported on quantification of lupeol by HPTLC, but the chemical compound was obtained from different species, such as Ficus (Rathee et al, 2015), Andrographis echioides leaves (Gurupriya et al, 2018; Kumar Ettaboina et al, 2022), and Alstonia scholaris (Ettaboina & Nakkala, 2021; Sanaye & Zehra, 2019). Although lupeol from various species was quantified using different HPTLC methods, not much data on the quantification of lupeol from Bauhinia species are available.…”

Section: Resultsmentioning

confidence: 99%

Development of a validated high‐performance thin‐layer chromatography method for quantification of lupeol from different parts of Bauhinia acuminata

Nakkala

Laddha

2022

Biomedical Chromatography

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The goal of the study was to determine how much lupeol was present in different Bauhinia acuminata samples. Lupeol was analyzed using a unique (verified) high‐performance thin‐layer chromatography (HPTLC) method. Separation of various compounds was achieved on silica gel (66 F 254) plates with toluene and methanol (99:1) as the mobile phase, followed by derivatization with Liebermann–Burchard reagent (r2 = 0.996). The proposed approach demonstrated a satisfactory linear relationship in the concentration range of 4–8 μg/mL. The limit of detection and limit of quantification were 63.84 ng/spot and 208.57 ng/spot, respectively. The proposed method was a simple and precise procedure that provided good resolution with other constituents of extracts. The validated HPTLC method described was simple, dependable, and practical and could be useful for standardizing and evaluating the quality of herbal material and formulations containing various Bauhinia species. The leaf, stem, unsaponified matter leaf, and stem petroleum ether extract had good amounts of lupeol (25.75, 38.25, 1.65, and 1.75, respectively). This research will be helpful for the quantitative determination of lupeol from herbal formulations as well as useful for further studies in this area.

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“…HPTLC studies of methanolic sample extract for identification of stigmasterol, quercetin, and kaempferol simultaneously in Indigofera tinctoria and quantification of kaempferol and quercetin in various medicinal plants has been performed [14,15]. Isolation and identification of stigmasterol and β-sitosterol through HPTLC from methanolic extract of root bark of Calotropis gigantea and quantification of stigmasterol and lupeol from Ficus religiosa [16,17] and lupeol from Andrographis echioides leaves has been done [18]. These types of quantification studies thus stand useful in suggesting a wide array of possible therapeutics from natural sources.…”

Section: Resultsmentioning

confidence: 99%

HPTLC METHOD FOR ESTIMATION AND QUANTIFICATION OF Β-Sitosterol FROM IN-VIVO AND IN-VITRO SAMPLES OF MERREMIA AEGYPTIA AND MERREMIA DISSECTA

Joshi

Meena

Mishra

et al. 2020

Asian J Pharm Clin Res

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Objective: A normal-phase high-performance thin-layer chromatography (HPTLC) method has been developed and validated for estimation and quantitation of beta-sitosterol from the methanolic fraction of different plant parts of two medicinally important plants viz. Merremia aegyptia and Merremia dissecta. These plants have been reported to possess antimicrobial, antioxidant, and anti-inflammatory activities. Methods: Chromatographic separation of beta-sitosterol from the methanolic extracts of plant parts of M. aegyptia and M. dissecta was performed on TLC aluminum plates pre-coated with silica gel 60F254 using a suitable mobile phase. The densitometric scanning was done after derivatization at ????-580 nm for ????-sitosterol. Result: Only M. dissecta leaf sample was reported to contain ????-sitosterol (4.6 ng/μl), whereas other samples such as seed, stem, and callus extracts of M. aegyptia and M. dissecta did not showed its presence. Conclusion: The developed HPTLC method is simple, rapid, and precise and can be used for routine analysis and quantification of ????-sitosterol and other useful plant bioactives that are phytopharmaceutically important.

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HPTLC Method for the Determination of Lupeol From Andrographis Echioides Leaves

Cited by 8 publications

References 15 publications

High-Performance Thin-Layer Chromatography Analysis and Free Radical Scavenging Potential of South Indian Orthodox Black Tea

High-Performance Thin-Layer Chromatography Analysis and Free Radical Scavenging Potential of South Indian Orthodox Black Tea

Development of a validated high‐performance thin‐layer chromatography method for quantification of lupeol from different parts of Bauhinia acuminata

HPTLC METHOD FOR ESTIMATION AND QUANTIFICATION OF Β-Sitosterol FROM IN-VIVO AND IN-VITRO SAMPLES OF MERREMIA AEGYPTIA AND MERREMIA DISSECTA

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