The indices of the content of proteins with molecular mass of 10-15 and 15-24 kDa in the plasmolemma of enterocytes of the small intestine of newborn calves in the dynamics from the birth till the age of 1 day were investigated. The study was performed on three groups of calves (the control group and two experimental ones) of Ukrainian black-and-white dairy breed by drawing the samples of enterocytes of jejunum and determining protein content of membrane lysate fraction by electrophoresis. The application of native liposomes based on soybean lecithin and "Membranostabil" medication for newborn calves with colostrum increases the expression of plasmolemic proteins of enterocytes with a molecular mass of 10-15 kDa. The dynamics of comparative analysis between fractions of protein expression with molecular mass of 10-15 and 15-24 kDa in the plasmolemma of the enterocytes of the jejunum of control and experimental groups of calves is shown. The result of application of native liposomes with phospholipid bilayer based on soybean lecithin and "Membranostabil" medication allows to assume the positive effect of these means on the level of colostral immunoglobulins in the serum of newborn calves.

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ЕКСПРЕСІЯ ІМУНОРЕЦЕПТОРНИХ ПРОТЕЇНІВ У ПЛАЗМОЛЕМІ ЕНТЕРОЦИТІВ НОВОНАРОДЖЕНИХ ТЕЛЯТ У ПЕРІОД ФОРМУВАННЯ КОЛОСТРАЛЬНОГО ІМУНІТЕТУ

The results of application of preparation "Membranostabil" and phospholipid-bilayer macrocapsules on the base of soybean lecithin developed by us with the aim of correction of indexes of content of immunoglobulin M in the blood of newborn calves during formation of colostral immunity is shown. The indexes of content of immunoglobulin M in the serum of blood of the newborn calves in dynamic were investigated-from birth till the 11 th day of life. The research was conducted on the three of groups of calves (the control one and two research) of Ukrainian black and white milk breed with drawing of blood samples from the jugular vein. Immunoglobulins M were examined by gel electrophoresis in a thin layer of a polyacrylamide gel. Quantitative estimation of protein fractions was carried out by scanning of the electrophoregrams with subsequent reconstructing of them graphically and calculating by relative units or area using a computer program. The results have proved that the preparation "Membranostabil" and phospholipid-bilayer macrocapsules based on soybean lecithin activate transport of immunoglobulins in small intestine and promote veracious increasing of the content of immunoglobulin M compared to the control group. The content of immunoglobulin M in the serum of newborn calves in both of the research groups at the age of 6 hours credibly increased and was higher during the whole period of the research except for calves of the 1 st research group at the age of 7 days compared to the calves of the control group. The dynamic with comparative analysis of content of immunoglobulin M in the serum of blood between different groups of calves of control and research groups is shown.The increasing of the content of immunoglobulin M in blood of newborn calves prevents development of digestive disorders in these animals.