Natural fibre reinforced polymer biocomposite as 2D laminate wound dressing material was developed and evaluated for its wound healing abilities. Two fibres, thuthi and banana were selected to construct a hybrid composite fibre (HCF). Both thuthi and HCF were reinforced with a polymer, poly vinyl alcohol (PVA) to develop a 2D laminate film material. Developed material was evaluated for its antibacterial activity and wound healing ability using a standard in vitro wound scratch assay method. Antibacterial activity showed potential results by exhibiting inhibitory zones against two bacterial wound causing pathogens, Escherichia coli and Staphylococcus aureus. The developed composite also showed promising wound healing abilities when assayed under in vitro conditions. The method implemented in this research to develop biocomposite shall be used for other fibres to optimize and determine the enhanced antibacterial actions and wound healing abilities. In future the natural fibre reinforced polymer biocomposite shall also be used in different forms and structures to meet the needs in the field of pharmaceutical science and medical textiles.