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The article focuses on the dependence of the retention of 3-glucosides of six common anthocyanidins: delphinidin, petunidin, malvidin, cyanidin, peonidin, and pelargonidin, -on the pressure in the column input, i.e. on the mobile phase flow rate. The study determined that on a 150×4.6 mm Symmetry C18 column, 3.5 µm, in the mobile phase of 10 vol.% of acetonitrile and 10 vol.% of formic acid in water, the retention increased with an increase in the mobile phase flow rate within the range of 0.2-0.8 cm 3 /min. An increase in the retention factor was maximal at the lowest temperature of separation. The increase in the retention factor was also proportional to the molar volume. For all the studied temperatures (30, 40, and 50 о С) the dependence of the natural logarithm of the retention factor of anthocyanins on the inlet pressure was described by a quadratic equation. This allowed us to calculate the enthalpy of transfer of anthocyanins from the mobile phase to the stationary phase using a traditional method, i.e. based on the natural logarithms of the retention factors with constant values of the mobile phase flow rate (i.e. different values of the inlet pressure) and natural logarithms of the calculated values of the retention factors of anthocyanins calculated using quadratic equations for the selected constant values of the inlet pressure (i.e. at different mobile phase flow rates). It appeared that in the first case the calculated enthalpy values for the same substances differed from the results of the calculations performed at different rates of mobile phase flow by about 10%. In the second case, the enthalpy values did not depend on the inlet pressure for all of the six anthocyanins. Therefore, to perform accurate calculation of the thermodynamic characteristics of sorbates, it is necessary to take into account the dependence of their retention on the mobile phase flow rate or the inlet pressure in the column.