We have proposed the design of high power microwave source in a vacuum tube based on the theory of negative mass instability. The proposed source does not operate based on a slow-wave structure but instead makes use of accelerated charged particles. In our structure, an electron emitter injects the charged particles into to the cavity. Emitted particles move around the anode, which leads to electron acceleration and emission of electromagnetic waves with a specific frequency. In this paper, we have enhanced the output power and frequency of microwave source by variation of particles initial energy and the number of anodes incorporating their specific arrangement and applied voltages. Working at room temperature, compactness, wide bandwidth, and tunability of output frequency are among the advantages of the proposed source. K: Accelerator modelling and simulations (multi-particle dynamics; single-particle dynamics); Instrumentation for particle accelerators and storage rings -low energy (linear accelerators, cyclotrons, electrostatic accelerators) 1Corresponding author.