Marine actinomycetes sediment samples were collected from Gulf of Mannar costal region, Kayalpatinam, located at Tuticorin district, Tamilnadu, India. Marine actinomycetes were isolated and evaluated for activity of L-asparaginase production. A total of 10 marine actinomycetes strains were isolated. Among 10 isolates, six were belongs to Streptomyces sp, three were belongs to Micromonospora sp and one was to Micropolyspora sp. Based on phenotypic characteristics, actinomycetes strains were screened for L-asparaginase production. Streptomyces sp KPMS5 and Micromonospora sp KPMS10 were showed large pink coloration on L-asparaginase production medium. The strains were further studied for maximum production and characterizations of culture condition of L-asparaginase enzyme were evaluated. Effect of substrate on L-asparaginase production was evaluated by enzyme assy. Maximum enzyme assay (1.4 mM) was observed on glucose followed by starch (1.12Mm) by Micromonospora sp KPMS10. In Streptomyces sp KPMS5 showed maximum of 1.25mM of enzyme assay on glucose substrate followed by lactose 1.17mM. Yeast extract was effectively used as substrate for maximum production of L-asparaginase by submerged fermentation. Further studies on purification and characterization are required to support the application of enzyme. The finding concludes isolates belongs to non-Streptomyces sp like Micromonospora sp is a potential novel source for L-asparaginase production.

This study was on isolation, purification and characterization of PHB from Vibrio mimicus strain isolated from sediment samples collected from Vellar estuary, Parangipettai. PHB produced from the cells that were grown at mass scale level under optimized growth parameters were used in the present study. The FT-IR analysis showed the presence of CH 2 -CH 3 -C=0 methyl esters in the extract and it also confirmed that the isolated product as PHB. Regarding degradation in the present work the complete bioplastic degradation i.e. 100% degradation was observed in 49 days at 35 o C, followed by 94% and 88% degradation at 30 o C and 25 o C respectively in 49 days of incubation whereas the least degradation was observed at 40 o C.