An efficient protocol was devised for rapid callus induction of Solanum nigrum Linn. from young leaves. MS medium supplemented with different concentrations IAA (1-3 mg/l) with BAP (0.5 mg/l) and NAA (1-3 mg/l) with BAP (0.5 mg/l) for callus initiation. The growth of the calli derived from leaves increased with time of incubation and remained almost constant after 30 days. For solasodine estimation, the field grown plant part of young leaves and in vitro callus (0.5 g each) were weighed and extracted thrice with methanol and subjected to HPLC. The solasodine content of field grown leaves extracts was 0.0798 mg g -1 whereas the solasodine content in the in vitro callus extracts were 0.142 mg g -1 in 2.5 mgL -1 IAA + 0.5 mgL -1 BAP, followed by 0.1162 mg g -1 in 2 mgL -1 NAA + 0.5 mgL -1 BAP.

Introduction. Here, we present a case of polymicrobial anaerobic spondylodiscitis. Case Presentation. A forty-five year-old female patient was referred to a specialist orthopaedic hospital with an eight week history of back pain without fevers. X-ray imaging and magnetic resonance imaging showed acute osteomyelitis of the twelfth thoracic and first lumbar vertebrae. Prolonged enrichment cultures grew Parvimonas micra and Fusobacterium nucleatum, identified by matrix-assisted laser desorption ionisation-time of flight (MALDI-ToF) mass spectrometry (MS). The patient was successfully treated with six weeks of intravenous ertapenem and oral clindamycin. Conclusion. Anaerobic discitis is rare, and polymicrobial discitis is rarer still. A PubMed literature review revealed only seven cases of F. nucleatum discitis and only twelve cases of P. micra discitis; this includes only one other reported case of a polymicrobial discitis due to infection with both anaerobes. We emphasise the importance of prolonging enrichment culture and the use of fast yet accurate identification of anaerobes using MALDI-ToF MS in these infections.

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