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Fertility of Red Chittagong bulls (RCB) using Non-Return Rate (NRR), reproductive efficiency of Red Chittagong cows (RCC) using pregnancy rate and number of services per pregnancy and parasitic infestation of two different ages and sexes were studied. The NRR ranged from 53.1% to 70.6% with an overall mean of 63.9%. Overall pregnancy rate was 65.8% and about 1.4 services were required per pregnancy. Overall 47.7% animals were infested with parasites. Five species of parasites were detected in the faeces: Balantidium coli (40%), Paramphistomum spp (47.7%), Fasciola spp (5.8%), Coccidia spp (4.5%), and Moniezia spp (1.9%). The prevalence of parasites in animals over four years age was higher (P<0.05) than in animals below four years of age: the prevalence was higher in female than in male hosts (P>0.05). (Bangl. vet.

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Rhizospheric bacteria Bacillus subtilis and Pseudomonas fluorescens are two widely tested biological control agents against root-knot nematodes (RKN) of different crops. However, their performance as bio-control agents varies with their place of origin. Culture filtrates of rhizospheric bacteria contain some intermediary metabolites that have nematicidal activity. An in vitro experiment was undertaken to evaluate the functionality of culture filtrates of B. subtilis (MN252542.1) and P. fluorescens (MN256394.1) at different concentrations (1.0%, 2.5%, 5.0%, 7.0%, 10.0% and 25.0%) on the hatching and mortality of Meloidogyne javanica at different time span. Bacterial strains were isolated from rhizospheric soils of Bangladesh. At three days after incubation (DAI), 25.0% concentration of culture filtrates of both B. subtilis and P. fluorescens showed 100.0% mortality of second stage juveniles (J 2 ) of M. javanica . Additionally, 25.0% concentration of culture filtrates of both bacteria showed 100.0% inhibition of hatching at one week after incubation (WAI). A decreasing trend in hatching of M. javanica was observed with the increment of the concentration of culture filtrates and progression of incubation time. The findings of this experiment reveal that culture filtrates of these accessions of B. subtilis and P. fluorescens are effective for controlling M. javanica and would be potential candidates for developing bio-nematicides.

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