Background Trichinellosis, a zoonosis caused by the genus Trichinella, is a widespread foodborne disease. Albendazole, one of the benzimidazole derivatives, is used for treating human trichinellosis, but with limited efficacy in killing the encysted larvae and numerous adverse effects. Cyperus rotundus L. is a herbal plant with a wide range of medicinal uses, including antiparasitic, and is frequently used in traditional medicine to treat various illnesses. Methods LC-ESI-MS was used to identify the active phytoconstituents in the methanol extract (MeOH ext.) of the aerial parts of C. rotundus and its derivate fractions ethyl acetate (EtOAc fr.), petroleum ether (pet-ether fr.), and normal butanol (n-BuOH fr.). The in vivo therapeutic effects of C. rotundus fractions of the extracts were evaluated using the fraction that showed the most promising effect after detecting their in vitro anti-Trichinella spiralis potential. Results C. rotundus extracts are rich in different phytochemicals, and the LC-ESI-MS of the 90% methanol extract identified 26 phenolic compounds classified as phenolic acids, flavonoids, and organic acids. The in vitro studies showed that C. rotundus extracts had a lethal effect on T. spiralis adults, and the LC50 were 156.12 µg/ml, 294.67 µg/ml, 82.09 µg/ml, and 73.16 µg/ml in 90% MeOH ext., EtOAc fr., pet-ether fr. and n-BuOH fr., respectively. The n-BuOH fr. was shown to have the most promising effects in the in vitro studies, which was confirmed by scanning electron microscopy. The in vivo effects of n-BuOH fr. alone and in combination with albendazole using a mouse model were evaluated by counting adults in the small intestine and larvae in the muscles, in addition to the histopathological changes in the small intestine and the muscles. In the treated groups, there was a significant decrease in the number of adults and larvae compared to the control group. Histopathologically, treated groups showed a remarkable improvement in the small intestine and muscle changes. Remarkably, maximal therapeutic effects were detected in the combination therapy compared to each monotherapy. Conclusion Accordingly, C. rotundus extracts may have anti-T. spiralis potential, particularly when combined with albendazole, and they may be used as synergistic to anti-T. spiralis medication therapy.

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Objectives:To study the detection rate of congenital fetal heart anomalies in first trimester scanning compared with second trimester scanning and to postnatal exam and neonatal echocardiography. Methods: This is a prospective observational study performed at a tertiary Fetal Medicine Unit. Patients had a first trimester scan from 11-14 weeks which included screening for Down's syndrome by measurement of the NT thickness, detection of Nasal bone, measurement of DV flow and tricuspid valve flow. Full anatomy exam was performed with special interest in the heart. Examination of the heart included; the four chamber view, intact inter-venticular septum, correct outflow tract and the three vessel view in the mediastinum. Pulsed Doppler was done at level of tricuspid valve to exclude regurgitation.A similar examination of the heart was performed at 20-24 weeks with full anatomy survey for other congenital malformations.Comparison of the two fetal heart examinations was done compared to final neonatal examination and neonatal echocardiography when indicated. Results: A total of 300 pregnant females were examined. The mean age of the patients were; 29.9 ± 6.3. Mean BMI was 32.5.The mean GA at the first trimester was 12.9 ± 0.9 and the mean GA at the second trimester was 20.4 ± 1.4.A total of 11 congenital heart anomalies were confirmed postnatally (3.7%).Seven were diagnosed and 4 were missed at the first trimester and one was falsely diagnosed as having an anomaly giving a detection rate of 63.6%, specificity 99.7%, PPV 87.5%, NPV 98.6% and agreement reached 98.3% (kappa 0.728)In the second trimester scan 9 cases were diagnosed, 2 cases were missed giving a detection rate of 81.8%, specificity 99%, PPV 75%, NPV 99.3% agreement 98.3% (kappa 0.774). Conclusions: First trimester heart examination has a good detection rate for congenital heart anomalies and should be done as a routine during first trimester screening for Down's syndrome. We describe here a remarkable case that had never been reported before. It's an antenatal diagnosis of a fistula between the rectum and the subcutaneous layer creating a meconial layer under the skin of the fetal bottom. Ultrasound and IRM showed this infiltration creating a mass extending to the caudal pole of the lumbosacral region at the root of both thighs. The surgical pediatric team confirmed the nature of the lesion after birth. Despite the important size of the mass, the pediatric and surgical cares have permitted an anatomic restitution. To our knowledge, such lesion has never been reported in the literature whether for an antenatal or a postnatal diagnosis. This case widens the range of perineal masses diagnosis. Teratoma of an undescended testicle is a rare cause of fetal abdominal cystic mass. The criteria for its prenatal diagnosis are unclear. We hereby present a prenatal diagnosis of isolated fetal testicular teratoma confirmed on early neonatal surgical excision, and describe two-and three-dimensional ultrasound, and Doppler criteria for its diagnosis. OP26.10A 31 year old ...

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