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After preparing an all-solid-state copper ion selective electrode (Cu 2+ -ISE) based on PEDOT/PSS conversion layer via electrodeposition, this paper characterizes the morphology and cyclic voltammetry performance of the PEDOT/PSS film, optimizes the deposition time of the PEDOT/PSS film, and tests the reproducibility, selectivity and acid-base resistance of the all-solid-state copper ion selective electrode. The experimental results show that the prepared all-solid-state copper ion selective electrode has a linear response in the range of 1×10 -6 mol•L -1 ~1×10 -2 mol•L -1 , with a response time less than 30 s, and the response slope of 27.8 mV/ decade. The all-solid-state copper ion selective electrode mentioned above can meet the detection standard of national II surface water.