Thymidine kinase 1 in serum (STK1) of patients with gastric cancer was determined by two methods: ECL dot blot and radioactivity assay. Both measurements showed significantly different values for preoperative STK1 and healthy STK1 (p=0.012 for ECL dot blot and p=0.003 for the radioactivity assay). The preliminary results of ECL dot blot STK1 measurement showed that in tumor-free subjects the level of the enzyme was significantly reduced to 52.7% 35 days after surgery (n=8, p=0.0106). The decrease in STK1 levels in the tumor-free subjects paralleled the decline of the half-life of the STK1 enzyme. In patients with distant metastases (n=6) the enzyme level had increased to 173% 35 days postoperatively. By contrast, with the radioactivity assay no significant differences in thymidine kinase activity for 0-day-postoperative patients and 35-day-postoperative tumor-free patients was found (p=0.329). The activity decreased to 80% in 35-day-postoperative patients with metastatic disease. We suggest that the value of the half-life of STK1 measured by ECL dot blot can be used as a potential marker for monitoring the response to surgery in patients with gastric or other cancers one month after surgery.

Thymidine kinase 1 in serum (STK1) of patients with gastric cancer was determined by two methods: ECL dot blot and radioactivity assay. Both measurements showed significantly different values for preoperative STK1 and healthy STK1 (p=0.012 for ECL dot blot and p=0.003 for the radioactivity assay). The preliminary results of ECL dot blot STK1 measurement showed that in tumor-free subjects the level of the enzyme was significantly reduced to 52.7% 35 days after surgery (n=8, p=0.0106). The decrease in STK1 levels in the tumor-free subjects paralleled the decline of the half-life of the STK1 enzyme. In patients with distant metastases (n=6) the enzyme level had increased to 173% 35 days postoperatively. By contrast, with the radioactivity assay no significant differences in thymidine kinase activity for 0-day-postoperative patients and 35-day-postoperative tumor-free patients was found (p=0.329). The activity decreased to 80% in 35-day-postoperative patients with metastatic disease. We suggest that the value of the half-life of STK1 measured by ECL dot blot can be used as a potential marker for monitoring the response to surgery in patients with gastric or other cancers one month after surgery.

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Ternary metal oxides containing Mn, Ni, and Co oxides were prepared according to the effortless hydrothermal avenue followed with calcination in air. The obtained nanohybrids (named MnNiCo2O4 NHBs) were applied as a positive material of asymmetric supercapacitors (ASCs) and as a cathode material of aqueous zinc-ion batteries (ZIBs). As a result, the MnNiCo2O4 NHBs showed very exciting energy storage performances and possessed a highest energy density of up to 45.4 W h kg–1 at a power density of 200 W kg–1 in an ionic liquid (IL) electrolyte. For comparison, the MnNiCo2O4 NHB ASCs with Na2SO4 electrolyte displayed a lower energy density of only ca. 19.9 W h kg–1 at 100 W kg–1, half the value of the MnNiCo2O4 NHB ASCs with ILs. Furthermore, the energy storage property of MnNiCo2O4 NHB-based aqueous ZIBs evidenced an excellent discharge capacity of 408 mA h g–1 at 0.1 A g–1 and a good rate performance of ca. 45.0 mA h g–1 even at 10 A g–1. Such a performance indicated that MnNiCo2O4 NHBs were a suitable bifunctional material for potential applications in the energy storage of ASCs and ZIBs.