"The study aimed to investigate to the spread areas of angular spotting disease on cucumber leaves caused by Pseudomonas syringae pv. Lachrymans In the greenhouses of three areas (Al-Qazwiynia , Abbasiya and Al-Haidariya) In the province of Najaf and the first time in Iraq to evaluate the efficiency of using chemical and biological induction agents to control angular spotting disease on cucumber leaves. The geographical distribution of the infected areas and the phenotypic diagnosis of symptoms were confirmed by field survey of the disease. It was later revealed that the diagnosed symptoms were similar to the symptoms of the disease, Where the rate of infection in plastic houses covered by the survey ranged between 19% to 35%. Results of the effect of biochemical and chemical agents on p. syringae pv. Lachrymans showed that treatment of P. putida was increased leaves content of chlorophyll, IAA and GA3 (93.28 mg.100g-1 , 1.61 µM and 24.02 µM, respectively) compared to control 2 treatment (44.65 mg.100g-1 , 0.84µM and 8.68 µM). Results showed that P. fluorescens was decresed ABA hormone which made plant health (324.19 µM) compared to Control 2 treatment (574.53 µM). The treatment of super fifty was superior in leaf content of carbohydrates, protein, CAT, POD, (28.25 mg.g-1 , 17.06%, 186.68 units.min.g-1 , 179.91 units.min.g-1) compared to control 2 treatment (13.19 mg.g-1, 8.56%, 58.16 units.min.g-1 , 105.90 units.min.g1). "