Background: Studies have shown different results on the association between fingerprints, gender and blood groups in the different settings. There is one published article in Sudan. Objectives: To investigate the prevalence of fingerprints characteristics and to assess if there is an association between fingerprints and gender or ABO blood groups and rhesus blood types in Sudan. Results: A total of 394 participants were enrolled in the study. Their age ranged between 19 to 24 years with a mean (standard deviation) of 21 (3) years. Of these 394, 114 (28.9%) and 280 (71.1%) participants were males and females, respectively. Of 394 participants, 111 (28.2%), 63 (15.9%), 13(3.3%), and 207(52.5%) had blood group A, blood group B, blood group AB and blood group O, respectively. While 373(94.7%) participants had rhesus positive blood, only 21 (5.3%) had rhesus negative blood. Of 394 fingerprints, 2476 (62.84%) were loop, 1278(32.44%) were arch and186 (4.72%) were whorls. Fingerprints were significantly between males and females and between the different blood groups and rhesus factors. Conclusion: loop, (62.84%) and arch (32.44%) were they common fingerprints. Fingerprints characteristics were significantly between males and females and between the different blood groups and Rhesus factor.