Irrigation water is a doorway for the pathogen contamination of fresh produce. We quantified pathogenic viruses [human adenoviruses, noroviruses of genogroups I and II, group A rotaviruses, Aichi virus 1 (AiV-1), enteroviruses (EnVs), and salivirus (SaliV)] and examined potential index viruses [JC and BK polyomaviruses (JCPyVs and BKPyVs), pepper mild mottle virus (PMMoV), and tobacco mosaic virus (TMV)] in irrigation water sources in the Kathmandu Valley, Nepal. River, sewage, wastewater treatment plant (WWTP) effluent, pond, canal, and groundwater samples were collected in September 2014, and in April and August 2015. Viruses were concentrated using an electronegative membrane-vortex method and quantified using TaqMan (MGB)-based quantitative PCR (qPCR) assays with murine norovirus as a molecular process control to determine extraction-reverse transcription-qPCR efficiency. Tested pathogenic viruses were prevalent with maximum concentrations of 5.5-8.8 log copies/L, and there was a greater abundance of EnVs, SaliV, and AiV-1. Virus concentrations in river water were equivalent to those in sewage. Canal, pond, and groundwater samples were found to be less contaminated than river, sewage, and WWTP effluent. Seasonal dependency was clearly evident for most of the viruses, with peak concentrations in the dry season. JCPyVs and BKPyVs had a poor detection ratio and correspondence with pathogenic viruses. Instead, the frequently proposed PMMoV and the newly proposed TMV were strongly predictive of the pathogen contamination level, particularly in the dry season. We recommend utilizing canal, pond, and groundwater for irrigation to minimize deleterious health effects and propose PMMoV and TMV as indexes to elucidate pathogenic virus levels in environmental samples.

Information regarding domestic water consumption is vital, as the Kathmandu Valley will soon be implementing the Melamchi Water Supply Project; however, updated information on the current situation after the 2015 Gorkha Earthquake (GEQ) is still lacking. We investigated the dynamics of domestic water consumption pre-and post-GEQ. The piped water supply was short, and consumption varied widely across the Kathmandu Upatyaka Khanepani Limited (KUKL) branches and altitude. The reduction in piped, ground, and jar water consumption and the increase in tanker water consumption post-GEQ appeared to be due to the impact of the GEQ. However, the impact did not appear to be prominent on per capita water consumption, although it was reduced from 117 to 99 L post-GEQ. Piped, ground, and tanker water use were associated with an increase and jar water use was associated with a decrease in water consumption. Despite improvements in quantity, inequality in water consumption and inequity in affordability across wealth status was well established. This study suggests to KUKL the areas of priority where improvements to supply are required, and recommends an emphasis on resuming performance. Policy planners should consider the existing inequity in affordability, which is a major issue in the United Nations Sustainable Development Goals.

Background: Depression and suicidal thinking occur frequently alongside HIV/AIDS, triggering profound detrimental impacts on quality of life, treatment adherence, disease progression, and mortality. Yet the psychosocial factors contributing to these psychiatric comorbidities remain underexplored, particularly in the developing country context. This study thus examined different dimensions of perceived family support in relation to depression and suicidal ideation among people living with HIV/AIDS (PLWHA) in Nepal.

Kathmandu Valley faces water scarcity from decades of the added burden of water storage and treatment, which imposed cost. We estimated the method-specific cost of in-house drinking water treatment (9 L/day used) based on equipment price and life, daily operation time, fuel used, and consumables replacement frequency, which were Nepalese Rupees (NRs) 23, 57, 392, 586 and 799 for chemicals, ceramic filter, boiling, Euro-Guard and reverse osmosis-ultraviolet (RO-UV) water purification, respectively. The monthly average water treatment cost was estimated based on these estimates and treatment methods used in households, obtained from a questionnaire survey of 1500 households, and its socioeconomic relationship in a Nepalese urban context was investigated. Of the households, 75% practiced at least one treatment method, (average, 1.4). The estimated monthly average cost per household was NRs 380. The fixed effects model showed that the cost was significantly higher in Lalitpur district, and in high school education households. Higher water insecurity perception of respondents was the main determinant of higher treatment cost, which was especially true in Lalitpur district. Water treatment added extra financial burden, especially for the poor households which should be averted or minimized by concerned authorities to provide adequate quantity, quality, and access to drinking water for all.

Wastewater-based epidemiology (WBE) is an approach that can be used to estimate COVID-19 prevalence in the population by detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater. As the WBE approach uses pooled samples from the study population, it is an inexpensive and non-invasive mass surveillance method compared to individual testing. Thus, it offers a good complement in low- and middle-income countries (LMICs) facing high costs of testing or social stigmatization, and it has a huge potential to monitor SARS-CoV-2 and its variants to curb the global COVID-19 pandemic. The aim of this review is to systematize the current evidence about the application of the WBE approach in mass surveillance of COVID-19 infection in LMICs, as well as its future potential. Among other parameters, population size contributing the fecal input to wastewater is an important parameter for COVID-19 prevalence estimation. It is easier to back-calculate COVID-19 prevalence in the community with centralized wastewater systems, because there can be more accurate estimates about the size of contributing population in the catchment. However, centralized wastewater management systems are often of low quality (or even non-existent) in LMICs, which raises a major concern about the ability to implement the WBE approach. However, it is possible to mobilize the WBE approach, if large areas are divided into sub-areas, corresponding to the existing wastewater management systems. In addition, a strong coordination between stakeholders is required for estimating population size respective to wastewater management systems. Nevertheless, further international efforts should be leveraged to strengthen the sanitation infrastructures in LMICs, using the lessons gathered from the current COVID-19 pandemic to be prepared for future pandemics.