Background:Menopause can have adverse effects on the quality of life of postmenopausal women. The main purpose of this study was to determine the Validity and Reliability of the Persian version of the Utian Quality of Life Scale (UQOL) in iranian postmenopausal women in 2019 Methods: The questionnaire was first translated into Persian. After its adaptation with the original version and backward translating it into English, the face and content validity were assessed by a group of experienced experts. To this end, exploratory factor analysis was performed by Principal Factor Analysis method with varimax rotation. Convergent validity was assessed by correlating the Persian version of UQOL and the 36-Item Short Form Health Survey (SF-36). Finally, the mean quality of life score of postmenopausal women in different domains as well as its mean in different domains based on age, education level, menopausal age, and number of children were calculated. Results: Regarding reliability, the alpha coefficient was obtained 0.66 for occupation domain, 0.52 for health domain, 0.50 for emotional domain, and 0.90 for sex domain. There was a significant relationship between quality of life and age, menopausal age, occupation and number of children in all domains in menopausal women. There was a significant relationship between BMI and quality of life score in postmenopausal women only in physical health domain. Conclusion:it seems this questionnaire can’t be used in research on the quality of life of postmenopausal women.